| Project/Area Number |
15K15719
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
兼平 正史 東北大学, 歯学研究科, 助教 (30177539)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | チタン / 骨再生 / マイクロ加工 / レーザー / プレス加工 / 歯周組織再生 / 歯周病 / インプラント / メンブレン / 骨増生 / スペースメイキング / 歯周組織再生治療 / 歯槽骨 / 純チタン / チタンメンブレン / レーザー加工 / Osteopontin |
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| Outline of Final Research Achievements |
In this study, we focus on the finding that cells grow on new inspired titanium membranes developed for tissue regeneration. A new cell culture method different from the conventional passage method that the cells are migrated and attached on the other media. We established a technology of cell culture amplification method in which cells could move between membranes. A osteoblast-like cell of MC3T3 was cultured on a porous titanium membrane having 20 μm pore size and through-hole structure with proximate span of 50 μm formed on a thin pure titanium sheet of 20 μm. Observation using GFP and analysis using immunostaining revealed the engraftment and proliferation of cells comparable to collagen - coated culture dishes even on pure titanium film. We will use this material to regenerate alveolar bone tissue.
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