Development of a bacteriophage therapy against periodontopathic microbiome
| Project/Area Number |
15K15773
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小島 美樹 大阪大学, 歯学研究科, 招へい教員 (20263303)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 口腔細菌叢 / 歯周病 / 歯学 / 微生物 / 代謝物質 / Filifactor alocis / Porphyromonas gingivalis / ビルレントファージ |
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| Outline of Final Research Achievements |
We performed this study with a periodontitis-associated microbe, Filfactor alocis, as a target. First, we tried to isolate a F. alocis-specific bacteriophage from culture supernatant acquired from F. alocis continuous culture. This experiment still continues today.
Next, we performed an amino acids profiling of F. alocis culture supernatant, to know the influence of F. alocis on oral microbiome dysbiosis by secreting metabolites into the environment. It was revealed that 10 L-amino acids （Asn, Ser, Gly, Ala, Pro, Val, Ile, Leu, Phe, Lys）and 4 D-amino acids were secreted by F. alocis. This finding will provide clues about dysbiosis in oral microbiome.
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Report
(3 results)
Research Products
(22 results)
