| Project/Area Number |
15K16514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Kyoto University (2016)
Tokyo University of Pharmacy and Life Science (2015) |
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Principal Investigator |
Takata Takumi 京都大学, 原子炉実験所, 特定准教授 (80379007)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | D-アミノ酸 / クリスタリン / 白内障 / 加齢 / タンパク質異常凝集 / 質量分析 / D-Asp / 水晶体 / crystallin / タンパク質 |
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| Outline of Final Research Achievements |
The lens α-crystallin is composed of two subunits αA- and αB-crystallin (αA-Crys and αB-Crys), which form various oligomers and complexes. Recent studies have shown that many aspartyl residues (Asp) in αA-Crys inverted to isomers (Lβ-Asp, Dα-Asp, Dβ-Asp) with age. It has not been well understood whether Asp isomers in native polymeric αA-Crys are different from those in dissociated αA-Crys or those in aggregated complexes. We therefore examined to clarify the distribution of Asp isomers in dissociated αA-Crys and those in aggregated αA-Crys. The results showed that the isomerization of Asp 58 in αA-Crys were highly detectable from the monomeric fraction. In addition, age-dependent Asp 58 isomerization in α-Crys is similar to the ratio from lens insoluble fraction. The isomerization of Asp as well as many other modifications would reduce the normal subunit-subunit interaction of α-Crys with aging, result in senile cataract formation.
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