| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We investigated the issue of whether the EMARS system with fluorescein tyramide (FT) is applicable to analysis of co-clustered molecules in the intracellular organelles. To this end, we constructed HRP or APEX targeted to the ER, Golgi and secretory vesicle. The expressed HRP or APEX in the intracellular organelles were co-localized with fluorescein labeling resulting from the EMARS reaction. These observations indicate that the EMARS reaction with FT as the labeling reagent is applicable to the analysis of co-clustered molecules in the intracellular organelles.
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