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Microglia Dependent Development of Neuronal Circuits in the Cerebellum

Research Project

Project/Area Number 15K18342
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionHiroshima University

Principal Investigator

Nakayama Hisako  広島大学, 医歯薬保健学研究院(医), 助教 (70397181)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsミクログリア / 小脳 / 神経回路形成 / 発達 / プルキンエ細胞 / 登上線維 / シナプス再編成 / シナプス刈り込み
Outline of Final Research Achievements

We investigated whether microglia was crucial for the development of cerebellar neuronal circuits We studied whether and how microglia contribute to climbing fiber (CF) synapse elimination in the developing cerebellum. By ablating microglia from the developing cerebellum pharmacologically and genetically, we found that microglia was needed for CF synapse elimination during the second postnatal week. We observed almost no sign of microglia engulfing CFs, suggesting that microglia contribute to CF synapse elimination in a mechanism other than phagocytosis. Instead, synapses which have been reported to be crucial for the CF synapse elimination were affected in transgenic mice with reduced microglia. These results suggest that microglia contribute to CF synapse elimination through regulating synaptic input to Purkinje cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive2016

    • Author(s)
      Matsumoto-Makidono, Y., Nakayama, H., Yamasaki, M., Miyazaki, T., Kobayashi, K., Watanabe, M., Kano, M., Sakimura, K., Hashimoto, K.
    • Journal Title

      Cell Reports

      Volume: 16 Issue: 4 Pages: 994

    • DOI

      10.1016/j.celrep.2016.06.053

    • URL

      https://pure.teikyo.jp/en/publications/3ce87e4a-7df7-4d28-8c16-070ceff28970

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CAPS1 RNA Editing Promotes Dense Core Vesicle Exocytosis.2016

    • Author(s)
      Miyake K, Ohta T, Nakayama H, Doe N, Terao Y, Oiki E, Nagatomo I, Yamashita Y, Abe T, Nishikura K, Kumanogoh A, Hashimoto K, Kawahara Y.
    • Journal Title

      Cell Reports

      Volume: 17 Issue: 8 Pages: 2004-2014

    • DOI

      10.1016/j.celrep.2016.10.073

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A CDC42EP4/septin-based perisynaptic glial scaffold facilitates glutamate clearance.2015

    • Author(s)
      Ageta-Ishihara N, Yamazaki M, Konno K, Nakayama H, Abe M, Hashimoto K, Nishioka T, Kaibuchi K, Hattori S, Miyakawa T, Tanaka K, Huda F, Hirai H, Hashimoto K, Watanabe M, Sakimura K, Kinoshita M.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 10090-10090

    • DOI

      10.1038/ncomms10090

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 神経細胞のResonance特性に関わるイオンチャネルの解析2017

    • Author(s)
      橋本浩一、槇殿佳子、中山寿子、山崎美和子、宮崎太輔、小林和人、渡辺雅彦、狩野方伸、崎村建司
    • Organizer
      第94回 日本生理学会大会
    • Place of Presentation
      浜松市
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genetic manipulation of mTORC1 signaling in mouse cerebellar Purkinje cell.2016

    • Author(s)
      Kassai, H., Sakai, Y., Nakayama, H., Maeda, T., Hashimoto, K., Kano, M., Aiba, A.
    • Organizer
      Neuroscience 2016 annual meeting Society for Neuroscience
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞膜Resonance特性に関わるイオンチャネル2016

    • Author(s)
      橋本浩一、槇殿佳子、中山寿子
    • Organizer
      第68回 日本生理学会 中国四国地方会
    • Place of Presentation
      岡山市
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Ion channels responsible for the resonant property of neurons in the inferior olive2016

    • Author(s)
      橋本浩一、中山寿子、松本佳子
    • Organizer
      第59回 日本神経化学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Spatial and temporal distribution of microglia and climbing fiber-Purkinje cell synapses during postnatal cerebellar development2015

    • Author(s)
      Nakayama Hisako, Chie Morimoto, Ryo Takimoto, Kouichi Hashimoto
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県・神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Functional roles of microglia in postnatal refinement of the cerebellar circuit2015

    • Author(s)
      Hisako Nakayama and Kouichi Hashimoto
    • Organizer
      新学術領域研究「グリアアセンブリによる脳機能発現の制御と病態」第2回夏の(国際)ワークショップ
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県・岡崎市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2018-03-22  

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