Imaging single synaptic vesicles at CNS presynaptic terminals
Project/Area Number |
15K18346
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Doshisha University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 開口放出 / 全反射蛍光顕微鏡 / イメージング / シナプス小胞 |
Outline of Final Research Achievements |
We used total internal reflection fluorescence microscopy at the live isolated calyx of Held terminal and visualized fusions and pre-fusion activities of single synaptic vesicles just beneath the plasma membrane. We also measured vesicle membrane and protein retrieval at the same time and in the same cell at the calyx of Held from rats and mice, and found that there may be two distinct modes of membrane and vesicle protein retrieval depending on the activity strength. We also found an important signaling pathway to couple vesicle membrane and vesicle protein uptakes.
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Report
(3 results)
Research Products
(7 results)