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Imaging single synaptic vesicles at CNS presynaptic terminals

Research Project

Project/Area Number 15K18346
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurophysiology / General neuroscience
Research InstitutionDoshisha University

Principal Investigator

Midorikawa Mitsuharu  同志社大学, 研究開発推進機構, 助教 (60632643)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords開口放出 / 全反射蛍光顕微鏡 / イメージング / シナプス小胞
Outline of Final Research Achievements

We used total internal reflection fluorescence microscopy at the live isolated calyx of Held terminal and visualized fusions and pre-fusion activities of single synaptic vesicles just beneath the plasma membrane.
We also measured vesicle membrane and protein retrieval at the same time and in the same cell at the calyx of Held from rats and mice, and found that there may be two distinct modes of membrane and vesicle protein retrieval depending on the activity strength. We also found an important signaling pathway to couple vesicle membrane and vesicle protein uptakes.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] マックスプランク実験医学研究所/マックスプランク生物物理化学研究所(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Distinct modes of endocytotic presynaptic membrane and protein uptake at the calyx of Held terminal of rats and mice.2016

    • Author(s)
      Okamoto Y, Lipstein N, Hua Y, Lin KH, Brose N, Sakaba T, Midorikawa M.
    • Journal Title

      eLife

      Volume: 5

    • DOI

      10.7554/elife.14643

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Imaging Exocytosis of Single Synaptic Vesicles at a Fast CNS Presynaptic Terminal.2015

    • Author(s)
      Midorikawa M, Sakaba T.
    • Journal Title

      Neuron

      Volume: 88 Issue: 3 Pages: 492-8

    • DOI

      10.1016/j.neuron.2015.09.047

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Live cell imaging of single synaptic vesicles at mammalian CNS presynaptic terminals.2017

    • Author(s)
      Midorikawa M
    • Organizer
      第94回日本生理学会大会シンポジウム
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamics of single synaptic vesicles at the presynaptic terminals of mammalian central synapses2016

    • Author(s)
      Mitsuharu Midorikawa and Takeshi Sakaba
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Calmodulin-Munc 13-1 signaling regulates the coordinated uptake of synaptic vesicle membrane and protein at the calyx of Held2016

    • Author(s)
      Yuji Okamoto, Takeshi Sakaba, and Mitsuharu Midorikawa
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-03-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Visualizing kinetics of single synaptic vesicles at the calyx-type presynaptic terminal.2015

    • Author(s)
      Mitsuharu Midorikawa and Takeshi Sakaba
    • Organizer
      第38回 日本神経科学大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-07-28
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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