| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Activation of hypusine pathway, which relates to the translation, contributes to malignant phenotype in cancer. However, since direct target genes of hypusine pathway remain unclear, I tried to identify mRNAs which bind to eIF5A, a translational factor, in this study.
As first, I performed RNA-ChIP assay for isolation of mRNAs, which were binding to eIF5A. After that, I analyzed its sequence using ion proton. Next, I and Dr. Tanimoto, a computational scientist, extracted the candidate genes. To evaluate whether the candidate genes bind to eIF5A, I performed RT-PCR using each specific primer of these genes. Then, I found that some candidate genes bound to eIF5A. In addition, to determine the pathways which are regulated by eIF5A, I performed KEGG pathway analysis against extracted candidate genes. As a result, I found that eIF5A might regulate the translation of cytoskeleton and adhesion molecules. Moreover, we tried to identify the binding motif of eIF5A based on RNA sequencing data.
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