| Project/Area Number |
15K18421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Nishida-Aoki Nao 国立研究開発法人国立がん研究センター, 研究所, 研究員 (80737114)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エクソソーム / 細胞外小胞 / Cre recombinase / 体内挙動 / Rapamycin / 乳がん |
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| Outline of Final Research Achievements |
The purpose of this study was to detect in vivo distribution of exosomes, which are an extracellular vesicle of about 100 nm diameter, secreted by cancer cells. The in vivo distribution of exosomes is expected to provide useful information to clarify the mechanism of promoting metastasis by cancer-derived exosomes. Because exosomes are about the same size as viruses, detection of delivery is difficult with current technologies. Therefore, in this study, we constructed an experimental system to detect delivery of exosomes derived from cancer cells by applying Cre-LoxP system. We developed technologies to increase the efficiency of the introduction of Cre recombinase into exosomes. However, we could not detect exosome delivery within this project.
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