| Project/Area Number |
15K18432
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Suzuki Rei 福島県立医科大学, 医学部, 助教 (80590546)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 膵癌 / microRNA / digital PCR / 抗がん薬 / 間質 / 線維化 / 化学療法 |
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| Outline of Final Research Achievements |
In this study, we utilized digital PCR to measure serum miRNA expression in pancreatic ductal adenocarcinoma (PDAC) patients. We firstly confirmed miRNAs which significantly altered expression in human pancreatic stellate cells (hPSC) co-cultured with PDAC cell lines compared with normal hPSC. We found that mir-328, mir-382 and mir-let-7d significantly altered its expression along with enhanced fibrosis-related gene expressions. Serum miRNA from PDAC patients and healthy controls was extracted and absolute quantitation of the miRNAs was performed with digital PCR. In comparison of expression level of 3 serum circulating miRNAs between 22 PDAC patients and 20 healthy controls, these 3 miRNAs could discriminate caner from controls and yielded area an under curve (AUC) of 0.76 for mir-328, 0.69 for mir-382 and 0.85 for mir-let-7d, respectively.We concluded that ｄigital PCR quantification of fibrosis-related miRNAs can be useful serum biomarkers for pancreatic ductal adenocarcinoma.
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