Structural Study of Influenza Virral NP and importin5 of Host Cell Factor.

• Project/Area Number: 15K18499
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Kanagawa Academy of Science and Technology
• Principal Investigator: Yoshida Hisashi 公益財団法人神奈川科学技術アカデミー, 革新的インフルエンザウイルス創薬プロジェクト, 研究員 (90724774)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: インフルエンザウイルス / X線結晶構造解析 / 核輸送 / 宿主因子 / 核移行 / NP

Outline of Final Research Achievements

In recent years, avian influenza infections in humans have been a large problem in the world. Influenza virus is able to grow effectively in the infectious cell using the host cell machinery. The three subunits of influenza RNA polymerase interact with the host cell importin family to move into the nucleus, then replication and transcription of viral gene is performed by RNA polymerase. NP which forms ribonucleoprotein cmplex with the viral RNA and RNA polymerase, is imported into the nucleus by the imporinα5. Although the nuclear localization of these proteins has been found to affect the growth efficiency of the virus, the details of molecular mechanisms such as the influence of the mutation is unclear. Crystallographic study was performed to resolve the interaction mechanism between NP and importinα5

Research Products

• [Journal Article] G196 epitope tag system: a novel monocle antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity (2017)
  • Author(s): Tatsumi K, Sakashita G, Nariai Y, Okazaki K, Kato H, Obayashi E, Yoshida H, Sugiyama K, Park SY, Sekine J, Urano T
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 43480-43480
  • DOI: 10.1038/srep43480
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5 (2017)
  • Author(s): Obayashi E, Luna RE, Nagata T, Martin-Marcos P, Hiraishi H, Singh CR, Erzberger JP, Zhang F, Arthanari H, Morris J, Pellarin R, Moore C, Harmon I, Papadopoulos E, Yoshida H, Nasr ML, Unzai S, Thompson B, Aube E, Hustak S, Stengel F, Dagraca E, Ananbandam A, Gao P, Urano T, Hinnebusch AG, Wagner G, Asano K.
  • Journal Title: Cell Report Volume: 18 Issue: 11 Pages: 2651-2663
  • DOI: 10.1016/j.celrep.2017.02.052
  • NAID: 120006358788
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit. (2015)
  • Author(s): Yoshida H, Park S-Y, Oda T, Akiyoshi T, Sato M, Shirouzu M, Tsuda K, Kuwasako K, Unzai S, Muto Y, Urano T, Obayashi E.
  • Journal Title: Genes Dev. Volume: 29 Issue: 15 Pages: 1649-1660
  • DOI: 10.1101/gad.267104.115
  • Peer Reviewed / Open Access