A research to reveal the cell-cell recognition mechanisms to maintain compartment boundaries

• Project/Area Number: 15K18536
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Tohoku University (2016-2017); Institute of Physical and Chemical Research (2015)
• Principal Investigator: Umetsu Daiki 東北大学, 生命科学研究科, 助教 (60620474)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: コンパートメント境界 / 組織構築 / 自己組織化 / 細胞の選り分け / 細胞間認識 / ショウジョウバエ / トランスクリプトーム解析

Outline of Final Research Achievements

The formation of straight compartment boundaries separating groups of cells is an evolutionarily conserved strategy for tissue growth and patterning during multicellular organism development. In this project, I aimed to reveal the mechanisms that underlie this phenomenon by considering as a fundamental process important for tissue organization by cellular self-organization. By using a recently developed genome editing technology, I found that a molecule, whose vertebrate homologues function in immune cells to recognize non-self molecules, plays an important role in maintaining compartment boundaries in a Drosophila epithelial tissue.

Research Products

• [Int'l Joint Research] Technische Universitat Dresden/Institute of Genetics(Germany)
• [Journal Article] Inference of Cell Mechanics in Heterogeneous Epithelial Tissue Based on Multivariate Clone Shape Quantification (2017)
  • Author(s): Tsuboi Alice、Umetsu Daiki、Kuranaga Erina、Fujimoto Koichi
  • Journal Title: frontiers in Cell and Developmental Biology Volume: 5 Pages: 1-16
  • DOI: 10.3389/fcell.2017.00068
  • Peer Reviewed / Open Access
• [Journal Article] Planar polarized contractile actomyosin networks in dynamic tissue morphogenesis (2017)
  • Author(s): Umetsu Daiki、Kuranaga Erina
  • Journal Title: Current Opinion in Genetics & Development Volume: 45 Pages: 90-96
  • DOI: 10.1016/j.gde.2017.03.012
  • Peer Reviewed / Open Access
• [Journal Article] Planar polarized contractile actomyosin networks in dynamic tissue morphogenesis (2017)
  • Author(s): Daiki Umetsu and Erina Kuranaga
  • Journal Title: Current Opinion in Genetics & Development Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A local difference in Hedgehog signal transduction increases mechanical cell bond tension and biases cell intercalations along the Drosophila anteroposterior compartment boundary (2015)
  • Author(s): Rudolf, K., Umetsu, D., Aliee, M., Sui, L., Julicher, F. and Dahmann, C
  • Journal Title: Development Volume: 142 Issue: 22 Pages: 3845-3858
  • DOI: 10.1242/dev.125542
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Differential expression of an ancient cell surface recognition molecule guides cell sorting at the anteroposterior compartment boundary in Drosophila (2018)
  • Author(s): Daiki Umetsu, Norihiro Iijima and Erina Kuranaga
  • Organizer: EMBO|EMBL Symposium: Tissue Self-Organization: Challenging the Systems
  • Int'l Joint Research
• [Presentation] Inference of cell mechanics in heterogeneous epithelial tissue based on multivariate clone shape quantification (2017)
  • Author(s): Daiki Umetsu, Alice Tsuboi, Koichi Fujimoto and Erina Kuranaga
  • Organizer: 4th Asia Pacific Drosophila Research Conference
  • Int'l Joint Research
• [Presentation] Inference of cell mechanics in heterogeneous epithelial tissue based on multivariate clone shape quantification (2017)
  • Author(s): Daiki Umetsu, Alice Tsuboi, Koichi Fujimoto and Erina Kuranaga
  • Organizer: 第2回 次世代生命科学の研究会
• [Presentation] A Link between Mechanical Control of Tissue Growth and Cell Competition (2016)
  • Author(s): Umetsu, D. and Kuranaga, E.
  • Organizer: 12th Japanese Drosophila Research Conference
  • Place of Presentation: 立教大学（東京都・豊島区）
  • Year and Date: 2016-09-09
• [Presentation] アクチン細胞骨格と原癌遺伝子Mycによる組織成長制御メカニズム (2016)
  • Author(s): 梅津 大輝
  • Organizer: 第一回 次世代生命科学の研究会
  • Place of Presentation: 徳島大学藤井節朗記念医科学センター（徳島県・徳島市）
  • Year and Date: 2016-08-12
• [Presentation] A link between mechanical control of tissue growth and cell competition (2016)
  • Author(s): Daiki Umetsu
  • Organizer: 1st Morphomeostasis Meeting
  • Place of Presentation: 国立遺伝学研究所（静岡県・三島市）
  • Year and Date: 2016-06-26
• [Presentation] PTP61Fの制御機構が細胞競合と細胞増殖の力学的制御のつながりを示唆する (2016)
  • Author(s): 梅津大輝、倉永英里奈
  • Organizer: 第五回 細胞競合コロキウム
  • Place of Presentation: 北海道大学遺伝子病制御研究所 (北海道 札幌市)
  • Year and Date: 2016-03-17
• [Presentation] Identification of mechanisms that protect cells from cell competition at anteroposterior compartment boundary in developing Drosophila wings (2015)
  • Author(s): Umetsu, D. and Kuranaga, E.
  • Organizer: 1st International Symposium on Cell Competition
  • Place of Presentation: 京都大学 (京都府 京都市)
  • Year and Date: 2015-09-10
  • Int'l Joint Research
• [Presentation] Tissue proliferation control by mechanical stresses (2015)
  • Author(s): Umetsu, D. and Kuranaga, E.
  • Organizer: The 26th CDB Meeting, Mechanistic Perspectives of Multicellular Organization
  • Place of Presentation: 理化学研究所多細胞システム形成研究センター (兵庫県 神戸市)
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 機械的ストレス応答による組織増殖の制御機構 (2015)
  • Author(s): 梅津大輝、倉永英里奈
  • Organizer: 第27回 高遠・分子細胞生物学シンポジウム
  • Place of Presentation: 延暦寺会館 (滋賀県 大津市)
  • Year and Date: 2015-08-26
• [Presentation] Local increases in mechanical tension guide cell sorting along compartment boundaries by biasing cell intercalations (2015)
  • Author(s): Daiki Umetsu
  • Organizer: 48th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際会議場 (茨城県 つくば市)
  • Year and Date: 2015-06-02
  • Int'l Joint Research / Invited
• [Remarks] Kuranaga Lab Website
  • URL: http://www.biology.tohoku.ac.jp/lab-www/kuranaga_lab/blog-3/publication.html