| Project/Area Number |
15K18552
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ジャスモン酸 / ジベレリン / 輸送体 |
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| Outline of Final Research Achievements |
Jasmonates (JAs) and gibberellins (GAs) have essential roles in regulating fertilization of Arabidopsis. We reported that GLUCOSINOLATE TRANSPORTER 1 (GTR1) expressed in Xenopus oocytes functions as a transporter for a bioactive form of JA and GA. We also show that gtr1 flowers have less bioactive GA, and the phenotype was complemented by the supplementation of bioactive GA, but not JA, suggesting GTR1 regulates fertility (Saito et al., 2015).
Here, we focused on the gene expression profiles, and found that gtr1 had some defect in normal GA biosynthesis and/or distribution in flowers. GTR2 is a close homolog of GTR1. We investigated phenotypes of gtr1, gtr2 and gtr1gtr2 double mutants, and found that gtr1gtr2 has shorter epidermal cells in filaments than that of WT. However, gtr1gtr2 had the same number of epidermal cells observed in WT. These results show that GTR1 and GTR2 have redundant functions in filament elongation, and such process is crucial to regulate timing of pollinizer.
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| Academic Significance and Societal Importance of the Research Achievements |
花粉の受粉のタイミングは植物ホルモンによって制御されている。本研究では花芽の発達時において植物ホルモンの１つジベレリンの輸送体として機能するGTR1およびその相同遺伝子GTR2の詳細な機能解析を行い、これらが花糸の伸長制御を介して受粉のタイミングを制御していることを示した。受粉が適切なタイミングで行われることは、作物の収量にも大きな影響を与える。そのため、受粉タイミングの制御機構の一端が明らかになることは、基礎研究のみならず、農業分野においても重要な知見となる。
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