| Project/Area Number |
15K18585
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | The Graduate University for Advanced Studies |
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Principal Investigator |
Shohei Takuno 総合研究大学院大学, 先導科学研究科, 助教(特定有期雇用) (20547294)
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| Research Collaborator |
Gaut Brandon S. University of California, Irvine, Department of Ecology and Evolutionary Biology, Professor
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ゲノム進化 / エピジェネティクス / 自然選択 |
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| Outline of Final Research Achievements |
DNA methylation is an important epigenetic modification that affects both chromatin packing and transcription. DNA methylation occurs in three sequence contexts, that is, CG, CHG, and CHH (where H is A, C, or T) in plants. All three contexts are methylated within repetitive elements. The major role of DNA methylation within repetitive elements is to silence transcription and functions as a host defense against transposons. On the other hand, only the CG context is mainly methylated within coding regions in plants that is called gene body methylation. Gene body methylation is considered a byproduct of the process of removing heterochromatic marks within active genes; gene body methylation might not be functional. However, I found that gene body methylation was observed in a biased subset of genes, tended to be conserved between plant orthologs, and the drastic changes of gene body methylation significantly affected expression levels.
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