Project/Area Number |
15K18629
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Science in genetics and breeding
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
AZUMA Akifumi 国立研究開発法人農業・食品産業技術総合研究機構, 果樹茶業研究部門, 上級研究員 (00391475)
|
Project Period (FY) |
2015-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | アントシアニン / ブドウ / エピゲノム / 着色 / 発現制御 / 遺伝子 / 植物 |
Outline of Final Research Achievements |
We investigated the mechanism of color mutation from red-skinned 'Benitaka' to black-skinned with red-fleshed 'Brazil'. The DNA methylation levels in the promoter region of VvMYBA1BEN allele in both cultivars were analyzed. Methylation levels of 'Benitaka' in skin and flesh were clearly higher than in that of 'Brazil' through the maturation stages. In addition, expression levels of VvMYBA1 and anthocyanin biosynthesis related genes were significantly higher in 'Brazil' for both skin and flesh than that of 'Benitaka'. These results suggest that the color mutation from 'Benitaka' to 'Brazil' was caused by the drastic decrease of DNA methylation level at the promoter region of VvMYBA1BEN. We concluded that this mutation accelrate the expression levels of anthocyanin biosynthesis related genes and anthocyanin accumulation in skin and flesh in 'Brazil'.
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Academic Significance and Societal Importance of the Research Achievements |
本研究は、ブドウのアントシアニン合成を制御する着色遺伝子座のDNAメチル化レベルの解析を着色変異個体を用いて行い、果皮アントシアニン含有量ならびに果肉着色化の新たな制御機構を明らかにした初めての例であり、学術的な意義が高い。また、本研究は温暖化に対応した優良着色品種の開発、ならびに果肉着色個体の効率的な作出に寄与できると期待されることから、今後の我が国におけるブドウ産業の活性化につながる成果として社会的意義も高い。
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