| Project/Area Number |
15K18644
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 植物ウイルス / RNAサイレンシング / RNAサイレンシングサプレッサー / 茎頂分裂組織 / アスパラガス / ウイルス / 種子伝染 / 免疫組織染色 / アスパラガスウイルス |
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| Outline of Final Research Achievements |
Asparagus virus 1 (AV-1) and Asparagus virus 2 (AV-2) are major viruses that infect asparagus. AV-1 is aphid transmission, while AV-2 is seed-borne virus. To study virus localization in asparagus, AV-1 and AV-2 were immunohistochemically examined. AV-1 and AV-2 were mainly detected in epidermal tissues and also detected in vascular tissues near meristem of lateral buds. AV-1 and AV-2 in flower buds were also examined immunohistochemically using male plants at different developmental stages. AV-2 was detected in entire bud tissues in all developmental stages and strongly detected in tapetum layer tissues at pollen development stage. AV-1 was not detected in earlier stage but detected after pollen tetrad stage, suggesting a possible seed transmission of AV-1. In addition, complete genome sequences of AV-1 isolated from asparagus spear were newly determined, and an infectious clone for AV-1 was developed and then confirmed systemic infection in Nicotiana benthamiana.
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