Genome editing with CRISPR/Cas system for genome-wide functional genomics in the rice blast fungus
| Project/Area Number |
15K18647
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Kobe University |
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Principal Investigator |
Arazoe Takayuki 神戸大学, 科学技術イノベーション研究科, 学術研究員 (40749975)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゲノム編集 / CRISPR/Cas9 / Target-AID / ポストゲノム / イネいもち病菌 / 麹菌 / 網羅的遺伝子破壊 / AID / CRISPR/Cas9 system / CRISPR/Cas system / 遺伝子ターゲティング / Pyricularia oryzae / 糸状菌 |
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| Outline of Final Research Achievements |
Simple and efficient functional gene analysis is a one of the most important issues in the post-genomics era. In this study, we exploited a fungal CRISPR/Cas9 system and applied genome editing to genome-wide functional genomics approaches in the rice blast fungus. Using this system, we established highly efficient gene targeting, multiple gene disruption, base modification, and reporter gene knock-in methods. In addition, we developed a novel genome-editing tool, “Target-AID”, which enables targeted nucleotide substitution without a DNA cleavage and donor gene by cytidine deamination. These genome-editing strategies can be used to expand various fundamental approaches and molecular breeding methods in filamentous fungi.
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant2017
Author(s)
Mizutani, O., Arazoe, T., Toshida, K., Hayashi, R., Ohsato, S., Skuma, T., Yamamoto, T., Kuwata, S. and Yamada, O.
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Journal Title
Journal of Biotechnology and Bioengineering
Volume: 3
Issue: 3
Pages: 287-293
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion2017
Author(s)
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T. and Kondo A.
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Journal Title
Nature Biotechnology
Volume: 印刷中
Issue: 5
Pages: 441-443
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems2016
Author(s)
Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K.Y., Shimatani, Z. and Kondo, A.
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Journal Title
Science
Volume: 353
Issue: 6305
Pages: 6305-6305
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus2015
Author(s)
Arazoe, T., Miyoshi, K., Yamato, T., Ohsato, S., Arie, T. and Kuwata, S.
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Journal Title
Biotechnology and Bioengineering
Volume: 112(12)
Issue: 12
Pages: 2543-2549
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 切らないゲノム編集技術2016
Author(s)
西田敬二，荒添貴之，島谷善平，坂野聡美
Organizer
日本植物学会第80回大会
Place of Presentation
沖縄コンベンションセンター (沖縄県・宜野湾市)
Year and Date
2016-09-16
Related Report
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