Research Project
Grant-in-Aid for Young Scientists (B)
Simple and efficient functional gene analysis is a one of the most important issues in the post-genomics era. In this study, we exploited a fungal CRISPR/Cas9 system and applied genome editing to genome-wide functional genomics approaches in the rice blast fungus. Using this system, we established highly efficient gene targeting, multiple gene disruption, base modification, and reporter gene knock-in methods. In addition, we developed a novel genome-editing tool, “Target-AID”, which enables targeted nucleotide substitution without a DNA cleavage and donor gene by cytidine deamination. These genome-editing strategies can be used to expand various fundamental approaches and molecular breeding methods in filamentous fungi.
All 2017 2016 2015 Other
All Int'l Joint Research (12 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 6 results, Open Access: 2 results, Acknowledgement Compliant: 3 results) Presentation (22 results) (of which Int'l Joint Research: 4 results, Invited: 4 results) Book (1 results) Patent(Industrial Property Rights) (2 results)
Journal of Biotechnology and Bioengineering
Volume: 3 Issue: 3 Pages: 287-293
10.1016/j.jbiosc.2016.09.014
40021152739
Nature Biotechnology
Volume: 印刷中 Issue: 5 Pages: 441-443
10.1038/nbt.3833
Bioresource Technology
Volume: 印刷中
Bioprotocol
Science
Volume: 353 Issue: 6305 Pages: 6305-6305
10.1126/science.aaf8729
Biotechnology and Bioengineering
Volume: 112(12) Issue: 12 Pages: 2543-2549
10.1002/bit.25662
