Analysis of the novel mode of action of fungicide "protein prenylation" that has been indicated by whole genome analysis

• Project/Area Number: 15K18655
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: The University of Shiga Prefecture
• Principal Investigator: Izumitsu Kosuke 滋賀県立大学, 環境科学部, 助教 (20579263)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 殺菌剤 / プレニル化 / ゲラニルゲラニルトランスフェラーゼ / トルニファニド / RHO型GTPase / 作用機構 / 耐性化機構 / 植物病原菌

Outline of Final Research Achievements

In our previous study, it was suggested that tolnifanide resistance gene encodes geranylgeranyl transferase (GGT1). In this study, we introduced the mutated GGT1 gene (C406Y) into the wild-type strain of Bipolaris maydis. The transformants expressing mutated GGT1 showed highly resistance to tolnifanide. We attempted gene knockout of GGT1 but no gene-disrupted strain was obtained, suggesting that GGT1 is essential gene in B. maydis. As a result of examining the localization of RHO1, predicted to undergo geranylgeranylation modification, it was suggested that tolnifanide may inhibit localization of RHO1.

Research Products

• [Journal Article] A serine/threonine kinase gene BcATG1 is involved in conidiation and sclerotial development in Botrytis cinerea. (2016)
  • Author(s): Sumita T., K. Izumitsu, C. Tanaka
  • Journal Title: Mycoscience Volume: 57 Issue: 2 Pages: 107-117
  • DOI: 10.1016/j.myc.2015.11.001
  • NAID: 40020763339
  • Peer Reviewed
• [Journal Article] Effects of calmodulin on expressionof lignin-modifyingenzymes in Pleurotus ostreatus (2014)
  • Author(s): Suetomi, T., Sakamoto, T., Tokunaga, Y., Kameyama, T.,Honda, Y., Kamitsuji, H., Kameshita, I., Izumitsu, K., Suzuki, K., Irie, T.
  • Journal Title: Curr. Genet. Volume: 14 Issue: 2 Pages: 1-14
  • DOI: 10.1007/s00294-014-0460-z
  • Peer Reviewed
• [Presentation] 全ゲノム解析による抗真菌性化合物 Tolnifanide 耐性遺伝子の同定 (2016)
  • Author(s): 重吉沙衣，入江俊一，鈴木一実，宮川 恒，田中千尋，泉津弘佑
  • Organizer: 第16回糸除菌分子生物学コンファレンス
  • Place of Presentation: 宇治市
  • Year and Date: 2016-11-17
• [Presentation] 抗真菌性化合物 Tolnifanide の選択毒性には GGTase-I の Cys221 が関与する (2016)
  • Author(s): 松原佳耶，吉田裕史，泉津弘佑，宮川 恒，田中千尋
  • Organizer: 第16回糸除菌分子生物学コンファレンス
  • Place of Presentation: 宇治市
  • Year and Date: 2016-11-17