| Project/Area Number |
15K18655
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | The University of Shiga Prefecture |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 殺菌剤 / プレニル化 / ゲラニルゲラニルトランスフェラーゼ / トルニファニド / RHO型GTPase / 作用機構 / 耐性化機構 / 植物病原菌 |
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| Outline of Final Research Achievements |
In our previous study, it was suggested that tolnifanide resistance gene encodes geranylgeranyl transferase (GGT1). In this study, we introduced the mutated GGT1 gene (C406Y) into the wild-type strain of Bipolaris maydis. The transformants expressing mutated GGT1 showed highly resistance to tolnifanide. We attempted gene knockout of GGT1 but no gene-disrupted strain was obtained, suggesting that GGT1 is essential gene in B. maydis. As a result of examining the localization of RHO1, predicted to undergo geranylgeranylation modification, it was suggested that tolnifanide may inhibit localization of RHO1.
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