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Interaction of hemicellulose with disordered regions along cellulose microfibril

Research Project

Project/Area Number 15K18723
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Horikawa Yoshiki  東京農工大学, (連合)農学研究科(研究院), 特任准教授 (90637711)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsセルロース / ヘミセルロース / ミクロフィブリル / 重合度 / エンドグルカナーゼ / 赤外分光分析
Outline of Final Research Achievements

For better biomass conversion, it is important to understand precise information about main components as well as interaction mechanism between these macromolecules. It has been accepted hemicelluloses interact with the surface of cellulose microfibril by hydrogen bonding. But strong alkaline treatment which is enough for cutting those linkages does not remove hemicellulose completely. We therefore verify other interaction of hemicellulose with cellulose microfibril by using endoglucanase which specifically hydrolyze amorphous region. Interestingly, cellulose DPs decreased with decrease of amounts of mannan and xylan, both of which are main components of softwood hemicellulose. The results obtained indicated hemicellulose partially interact with disordered regions existed along cellulose microfibril.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Visualization of cellulase interactions with cellulose microfibril by transmission electron microscopy2017

    • Author(s)
      Horikawa Y., Imai T., Sugiyama
    • Journal Title

      Cellulose

      Volume: 1 Issue: 1 Pages: 1-9

    • DOI

      10.1007/s10570-016-1105-9

    • NAID

      40021492328

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Assessment of endoglucanase activity by analyzing the degree of cellulose polymerization and high-throughput analysis by near-infrared spectroscopy2016

    • Author(s)
      Horikawa Y., Imai T., Abe K., Sakakibara K., Tsujii Y., Mihashi A., Kobayashi Y., Sugiyama J.
    • Journal Title

      Cellulose

      Volume: 23 Issue: 3 Pages: 1565-1572

    • DOI

      10.1007/s10570-016-0927-9

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of Pinus species related to historic architecture in Korea using NIR chemometric approaches2016

    • Author(s)
      Sung-Wook Hwang, Yoshiki Horikawa, Won-Hee Lee, Junji Sugiyama
    • Journal Title

      Journal of Wood Science

      Volume: 62 Issue: 2 Pages: 156-167

    • DOI

      10.1007/s10086-016-1540-0

    • NAID

      120005954465

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of thermochemical pretreatment on lignin alteration and cell wall microstructural degradation in Eucalyptus globulus: comparison of acid, alkali, and water pretreatments2016

    • Author(s)
      Kaori Saito, Yoshiki Horikawa, Junji Sugiyama, Takashi Watanabe, Yoshinori Kobayashi, Keiji Takabe
    • Journal Title

      Journal of Wood Science

      Volume: 印刷中 Issue: 3 Pages: 276-284

    • DOI

      10.1007/s10086-016-1543-x

    • NAID

      40020853015

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chemometrics approach for species identification of Pinus densiflora sieb. et Zucc. and Pinus densiflora for. erecta Uyeki.2015

    • Author(s)
      Sung-Wook Hwang, Won-Hee Lee, Yoshiki Horikawa, Junji Sugiyama
    • Journal Title

      Journal of Korean Wood Science and Technology

      Volume: 43 Issue: 6 Pages: 701-713

    • DOI

      10.5658/wood.2015.43.6.701

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] スギから誘導したカルスのセルロースミクロフィブリルにおける長さ方向の高次構造2017

    • Author(s)
      堀川祥生,半 智史,船田 良,山岸祐介,斎藤継之,磯貝 明,杉山淳司
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      九州大学箱崎キャンパスならびにアクロス福岡(福岡県福岡市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] セルロースミクフィブリの長さ方向における高次構造2016

    • Author(s)
      堀川祥生,清水美智子,斎藤継之,磯貝明,今井友也,杉山淳司
    • Organizer
      第23回セルロース学会年次大会
    • Place of Presentation
      つくばカピオ(茨城県つくば市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水生藻類シャジクモセルロースにおける長さ方向の高次構造2016

    • Author(s)
      堀川祥生、清水美智子、斎藤継之、磯貝明、今井友也、杉山淳司
    • Organizer
      第66回日本木材学会大会
    • Place of Presentation
      名古屋大学 全学教育棟・豊田講堂(名古屋市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] Cellulose synthase activity assayed in the living cell2016

    • Author(s)
      Shi-jing Sun, Yoshiki Horikawa, Junji Sugiyama, Tomoya Imai
    • Organizer
      ACS Spring Meeting 2016
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-03-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Longitudinal order on cellulose microfibrils in aquatic algae2016

    • Author(s)
      Yoshiki Horikawa, Tomoya Imai, Junji Sugiyama
    • Organizer
      ACS Spring Meeting 2016
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-03-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 水生藻類シャジクモセルロースの長さ方向における高次構造2015

    • Author(s)
      堀川祥生、今井友也、杉山淳司
    • Organizer
      セルロース学会第22回年次大会
    • Place of Presentation
      北海道大学 学術交流会館(札幌市)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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