Analysis of genetic diversity and host-specificity of non-primate hepacivirus

• Project/Area Number: 15K18779
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: University of Yamanashi
• Principal Investigator: TANAKA Tomohisa 山梨大学, 大学院総合研究部, 特任助教 (30585310)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: へパシウイルス / 翻訳 / ゲノム複製 / 多様性 / 非翻訳領域 / ヘパシウイルス / 疫学 / ウイルス / 翻訳開始 / C型肝炎ウイルス

Outline of Final Research Achievements

Non-primate hepacivirus (NPHV) is thought to share the ancestor with hepatitis C virus (HCV), which is an important viral model for elucidating the origin, pathogenicity or host-tropism of HCV. However, the epidemiological or virological evidence is still poor. In this study, we isolated NPHV from some endemic species in Japan and phylogenetically analyzed them in order to elucidate the genetic diversity of NPHV. Moreover, we investigated the mechanisms of equine hepacivirus (EHcV) 5’ UTR in IRES-dependent translation initiation and the association of EHcV 5’ UTR with miR-122-indepdendent viral replication.

Research Products

• [Journal Article] Roles of the 5' Untranslated Region of Nonprimate Hepacivirus in Translation Initiation and Viral Replication. (2018)
  • Author(s): Tanaka T, Otoguro T, Yamashita A, Kasai H, Fukuhara T, Matsuura Y, Moriishi K.
  • Journal Title: Journal of Virology Volume: 92 Issue: 7
  • DOI: 10.1128/jvi.01997-17
  • Peer Reviewed
• [Journal Article] Seroepidemiology of non-primate hepacivirus (NPHV) in Japanese native horses (2018)
  • Author(s): Hayashi Shizuka、Tanaka Tomohisa、Moriishi Kohji、Hirayama Kazuhiro、Yamada Akio、Hotta Kozue
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 1 Pages: 186-189
  • DOI: 10.1292/jvms.17-0527
  • NAID: 130006331060
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Presentation] 国内におけるげっ歯類へパシウイルスの遺伝的特徴とへパシウイルス非翻訳領域の機能比較 (2018)
  • Author(s): 田中智久
  • Organizer: 第6回肝炎ウイルス研修会
• [Presentation] 5’-terminal stem-loop of equine hepacivirus RNA permits miR-122-independent replication (2017)
  • Author(s): Tomohisa Tanaka, Teruhime Otoguro, Kaori Okuyama-Dobashi, Hirotake Kasai, Atsuya Yamashita, Kohji Moriishi
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Cross-species compatibility of hepacivirus 5’ untranslated region (2016)
  • Author(s): Tomohisa Tanaka, Teruhime Otoguro, Hirotake Kasai, Atsuya Yamashita, Kaori Okuyama-Dobashi and Kohji Moriishi
  • Organizer: 日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌）
  • Year and Date: 2016-10-23
• [Presentation] Functional homology of hepacivirus 5’ UTRs in IRES activity and replication (2016)
  • Author(s): Tomohisa Tanaka, Teruhime Otoguro, Atsuya Yamashita, Hirotake Kasai, and Kohji Moriishi
  • Organizer: 23rd International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: 京都国際会館（京都府・京都市）
  • Year and Date: 2016-10-11
  • Int'l Joint Research
• [Presentation] ウマヘパシウイルスが有するIRES活性の特徴 (2015)
  • Author(s): 田中智久、陳文家、乙黒光姫、葛西宏威、山下篤哉、森石恆司
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡県・福岡市
  • Year and Date: 2015-11-22
• [Presentation] Characterization of equine hepacivirus 5’ UTR regarding IRES activity (2015)
  • Author(s): 田中智久、森石恆司
  • Organizer: 22nd International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2015-10-09
  • Int'l Joint Research