| Project/Area Number |
15K18795
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Nihon University |
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Principal Investigator |
OGUMA Keisuke 日本大学, 生物資源科学部, 講師 (50436804)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 猫コロナウイルス / ウイルスゲノム / 変異解析 / 遺伝子診断 / 猫伝染性腹膜炎 / 遺伝子変異 / ウイルス遺伝子の変異 / ウイルス病原性の変化 / スパイク遺伝子 / 3c遺伝子 / 7遺伝子 / 猫伝染性腹膜炎ウイルス / 病原性転換 |
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| Outline of Final Research Achievements |
Feline coronavirus (FCoV) is classified into two types based on its pathogenicity in cats: a feline enteric coronavirus (FECV) causing mild diarrhea and a feline infectious peritonitis (FIP) virus (FIPV) that is lethal. The difference of FECV and FIPV has been considered to be attributed to the discrepancy of one or more of viral genes. It had been discovered that about 95% FIPVs had a mutation at specific position in the S gene of FCoV. Therefore, some commercial veterinary laboratories have offered the tests for the diagnosis of FIP through the analysis of S gene sequence to distinguish FIPVs from FECVs in the clinical specimens. Another candidate is 3c gene, which has been reported to have one or more mutations in more than 60% FIPVs. Because the relationship between the mutation of S and 3c genes was not well studied, we analysed these genes of FCoVs detected in clinical specimens such as ascites, tissues, and feces of cats.
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