Positional cloning and functional analysis of the non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1
Project/Area Number |
15K18807
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Insect science
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Research Institution | Tokyo University of Agriculture and Technology |
Principal Investigator |
Ito Katsuhiko 東京農工大学, (連合)農学研究科(研究院), 助教 (80725812)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | カイコ / 濃核病ウイルス / ウイルス抵抗性遺伝子 / ポジショナルクローニング / トランスクリプトーム解析 / ウイルス / 抵抗性遺伝子 / アミノ酸置換 / 抵抗性 / 遺伝子 / 次世代シークエンサー |
Outline of Final Research Achievements |
The aims of this study are to isolate two non-susceptibility genes to Bombyx mori densovirus (BmDV), Nid-1 and nsd-1, and clarify the mechanisms of absolute resistance of B. mori to BmDV. To identify Nid-1, we performed transcriptome analysis using total RNA purified from the midgut of the virus-inoculated and virus-non-inoculated silkworms strain caring Nid-1. RNA-seq analysis revealed clear difference in the expression levels in many genes between the virus-inoculated and virus-non-inoculated silkworm, however, there was no gene which is mapped to the region responsible for Nid-1. On the other hand, we succeeded to identify the gene responsible for nsd-1. Sequence and biochemical analyses revealed that nsd-1 encodes a Bombyx-specific mucin-like glycoprotein with a single transmembrane domain. Moreover, the NSD-1 protein was specifically expressed in the larval midgut epithelium, the known infection site of BmDV.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus2018
Author(s)
Ito K, Kidokoro K, Katsuma S, Sezutu H, Uchino K, Kobayashi I, Tamura T, Yamamoto K, Mita K, Shimada T, Kadono-Okuda K
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Journal Title
Scientific reports
Volume: 印刷中
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Peer Reviewed / Open Access
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[Book] ホルモンから見た生命現象と進化シリーズ VII 生体防御・社会性2016
Author(s)
伊藤 克彦, 岩室 祥一, 岩田 惠理, 和泉 俊一郎, 亀谷 美恵, 加藤 貴大, 小林 哲也, 近藤 朱音, 倉田 祥一朗, 沓掛 展之, 三浦 徹, 水澤 寛太, 向井 秀仁, 棟方 有宗, 中村 修, 新美 輝幸, 高橋 明義, 竹村 明洋, 竹内 栄, 竹内 悠記, 筒井 繁行, 上田 直子, 矢田 崇
Total Pages
280
Publisher
裳華房
Related Report