| Project/Area Number |
15K18807
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Ito Katsuhiko 東京農工大学, (連合)農学研究科(研究院), 助教 (80725812)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | カイコ / 濃核病ウイルス / ウイルス抵抗性遺伝子 / ポジショナルクローニング / トランスクリプトーム解析 / ウイルス / 抵抗性遺伝子 / アミノ酸置換 / 抵抗性 / 遺伝子 / 次世代シークエンサー |
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| Outline of Final Research Achievements |
The aims of this study are to isolate two non-susceptibility genes to Bombyx mori densovirus (BmDV), Nid-1 and nsd-1, and clarify the mechanisms of absolute resistance of B. mori to BmDV. To identify Nid-1, we performed transcriptome analysis using total RNA purified from the midgut of the virus-inoculated and virus-non-inoculated silkworms strain caring Nid-1. RNA-seq analysis revealed clear difference in the expression levels in many genes between the virus-inoculated and virus-non-inoculated silkworm, however, there was no gene which is mapped to the region responsible for Nid-1. On the other hand, we succeeded to identify the gene responsible for nsd-1. Sequence and biochemical analyses revealed that nsd-1 encodes a Bombyx-specific mucin-like glycoprotein with a single transmembrane domain. Moreover, the NSD-1 protein was specifically expressed in the larval midgut epithelium, the known infection site of BmDV.
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