TMEPAI family inhibits proliferation of malignant mesothelioma cells.

• Project/Area Number: 15K18866
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Showa Pharmaceutical University
• Principal Investigator: Nakano Naoko 昭和薬科大学, 薬学部, 助教 (50733218)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: C18ORF1 / YAP / TMEPAI family / Hippo pathway / TGF-βsignal / TMEPAI

Outline of Final Research Achievements

Integration between TGF-b and YAP signaling pathways has been reported in malignant mesothelioma (MM) cells. We found that YAP signal could be negatively controlled by TMEPAI family which we have already identified as a negative regulator of TGF-b signaling. In addition, we identified that the PY motifs in TMEPAI family play an important role in their interaction with YAP via its WW domains. We also found that TMEPAI family inhibits proliferation of MM cells.

Research Products

• [Journal Article] TMED10 protein interferances with transforming growth factor-β signaling by disrupting TGF-β receptor complex formation (2017)
  • Author(s): Naoko Nakano, Yuki Tsuchiya, kenro Kako, Kenryu Umezaki, Keigo Sano, Souichi Ikeno, Eri Otsuka, Masashi Shigeta, Ai Nakagawa, Nobuo Sakata, Fumiko Itoh, Yota Nakano, Shun-ichiro Iemura, Maarten van Dinther, Tohru Natsume, Peter teb Dijke and Susumu Itoh
  • Journal Title: The joural of biological chemistry Volume: 292 Pages: 4099-4112
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Regulation of the TMEPAI promoter by TCF7L2: the C-terminal tail of TCF7L2 is essential to activate the TMEPAI gene (2016)
  • Author(s): Naoko Nakano, Mitsuyasu Kato & Susumu Itoh
  • Journal Title: The journal of biochemistry Volume: 159 Issue: 1 Pages: 27-30
  • DOI: 10.1093/jb/mvv117
  • NAID: 120007129718
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] TMED10はTGF-β受容体複合体形成を抑制しTGF-βシグナル伝達を阻害する (2016)
  • Author(s): 中野なおこ、土屋裕樹、加古拳朗、梅嵜乾隆、佐野圭吾、池野聡一、大塚愛理、中川愛、坂田宣夫、伊東史子、家村俊一郎、夏目徹、伊東進
  • Organizer: 第39回 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 (神奈川県 横浜市)
  • Year and Date: 2016-11-30
• [Presentation] Establishment of protein knockdown specific for transcriptional co-activator YAP (2016)
  • Author(s): 中野なおこ、服部隆行、内藤幹彦、伊東進
  • Organizer: 第75回 日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜 (神奈川県 横浜市）
  • Year and Date: 2016-10-06
• [Presentation] がん遺伝子YAPを標的としたプロテインノックダウン法の確立 (2016)
  • Author(s): 中野なおこ、正田卓司、服部隆行、栗原正明、内藤幹彦、伊東進
  • Organizer: 第20回 日本癌分子標的治療学会学術集会
  • Place of Presentation: 別府国際コンベンションセンター (大分県 別府市)
  • Year and Date: 2016-05-30
• [Presentation] TMED10 dissociates TGF-beta receptor complex to inhibit TGF-beta signal (2015)
  • Author(s): Naoko Nakano
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場 (名古屋市)
  • Year and Date: 2015-10-08