Physiological and pathophysiological roles of an orphan G protein-coupled receptor

• Project/Area Number: 15K18867
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Teikyo University
• Principal Investigator: TANIKAWA TAKASHI 帝京大学, 薬学部, 助教 (00609985)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 脂質生化学 / リゾリン脂質 / Gタンパク質共役型受容体 / リゾホスファチジルイノシトール / GPR55

Outline of Final Research Achievements

GPR55 is an orphan G protein-coupled receptor and a putative novel type of cannabinoid receptor. Lysophosphatidylinositol (LPI) is an endogenous ligand for GPR55. It has been reported that GPR55 mRNA expresses in immune system. This study revealed that GPR55 mRNA expression was increased with the progression of maturation of T cells in thymus and was high in T cells in spleen compared to thymus. The level of GPR55 mRNA in B cells derived from bone marrow was higher than those from spleen. The level of GPR55 mRNA changed when the cells were activated. LPI promoted the cell proliferation. It seems that GPR55 and LPI of its endogenous ligand modulate the cell proliferation, cell differentiation and immune response in immune system.

Research Products

• [Journal Article] Coenzyme-A-Independent Transacylation System; Possible Involvement of Phospholipase A2 in Transacylation. (2017)
  • Author(s): Yamashita A, Hayashi Y, Matsumoto N, Nemoto-Sasaki Y, Koizumi T, Inagaki Y, Oka S, Tanikawa T, Sugiura T.
  • Journal Title: Biology (Basel) Volume: 印刷中 Issue: 2 Pages: 23-23
  • DOI: 10.3390/biology6020023
  • Peer Reviewed / Open Access
• [Journal Article] Carboxyl-terminal Tail-mediated Homodimerizations of Sphingomyelin Synthases Are Responsible for Efficient Export from the Endoplasmic Reticulum. (2017)
  • Author(s): Hayashi Y, Nemoto-Sasaki Y, Matsumoto N, Tanikawa T, Oka S, Tanaka Y, Arai S, Wada I, Sugiura T, Yamashita A.
  • Journal Title: J Biol Chem. Volume: 292 Issue: 3 Pages: 1122-1141
  • DOI: 10.1074/jbc.m116.746602
  • Peer Reviewed / Open Access
• [Journal Article] Myeloid-Derived Suppressor Cells Endow Stem-like Qualities to Breast Cancer Cells through IL6/STAT3 and NO/NOTCH Cross-talk Signaling. (2016)
  • Author(s): Peng D, Tanikawa T, Li W, Zhao L, Vatan L, Szeliga W, Wan S, Wei S, Wang Y, Liu Y, Staroslawska E, Szubstarski F, Rolinski J, Grywalska E, Stanislawek A, Polkowski W, Kurylcio A, Kleer C, Chang AE, Wicha M, Sabel M, Zou W, Kryczek I.
  • Journal Title: Cancer Res. Volume: 76 Issue: 11 Pages: 3156-3165
  • DOI: 10.1158/0008-5472.can-15-2528
  • Peer Reviewed / Int'l Joint Research
• [Presentation] マウスリンパ球におけるGタンパク質共役型受容体GPR55の機能 (2016)
  • Author(s): 谷川 尚、岡 沙織、中島圭佑、山下 純、杉浦隆之
  • Organizer: 第89回 日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] GPR55リガンドとしての各種1-アシル型および2-アシル型リゾホスファチジルイノシトール(LPI)の生物活性の比較 (2016)
  • Author(s): 岡 沙織、谷川 尚、山下 純、渡邊真知子、杉浦隆之、中島圭佑
  • Organizer: 第89回 日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-09-25
• [Presentation] Gタンパク質共役型受容体GPR55のマウス免疫系細胞における発現及び機能について (2016)
  • Author(s): 谷川 尚、岡 沙織、中島圭佑、山下 純、杉浦隆之
  • Organizer: 第136年会 日本薬学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-26
• [Presentation] 各種1-アシル型および2-アシル型リゾホスファチジルイノシトール(LPI)の調製と生物活性の比較 (2016)
  • Author(s): 中島圭佑、谷川 尚、山下 純、渡邊真知子、杉浦隆之、岡 沙織
  • Organizer: 第136年会 日本薬学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-03-26
• [Presentation] マウスT細胞及びB細胞におけるGタンパク質共役型受容体GPR55の発現 (2015)
  • Author(s): 谷川 尚、岡 沙織、中島圭佑、山下 純、杉浦隆之
  • Organizer: 第88回 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] GPR55 and its endogenous ligand (2015)
  • Author(s): Takashi Tanikawa, Saori Oka, Takayuki Sugiura
  • Organizer: Cannabinoid Function in the CNS Gordon Research Conference
  • Place of Presentation: Renaissance Tuscany Il Ciocco (Lucca (Barga), Italy)
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Remarks] 帝京大学ホームページ教員紹介
  • URL: https://www.e-campus.gr.jp/staffinfo/public/staff/detail/2093/16