| Project/Area Number |
15K18945
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Fukuoka University |
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Principal Investigator |
Hazekawa Mai 福岡大学, 薬学部, 助教 (10509186)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ミセル / PLGA / 卵巣がん / Glypican-3 / siRNA / 腹膜播種モデル / ＰＬＧＡ / 子宮頸がん |
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| Outline of Final Research Achievements |
It was recently reported that Glypican-3 (GPC-3) was specifically overexpressed in ovarian cancer. We focused on GPC-3 gene silencing for ovarian cancer therapy employing a siRNA delivery system. The purpose of our study was to prepare self-assembled siRNA-poly (D,L-lactic-co-glycolic acid) (PLGA) hybrid conjugate micelles for GPC-3 gene silencing. For in vivo experiments, micelles were administrated intraperitoneally in a mouse model of peritoneal dissemination. At 14 days after treatment with micelles, micelles decreased the volume of peritoneal fluid and the number of tumor nodes in the mesentery, compared with in the control. Self-assembled siRNA-PLGA hybrid conjugate micelles are, thus, potentially useful as an efficient siRNA delivery system for ovarian cancer therapy.
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