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Development of glycosylated lipid metabolomics system for clarifying their neuronal function

Research Project

Project/Area Number 15K19016
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionFujita Health University (2016)
Institute of Physical and Chemical Research (2015)

Principal Investigator

Nakajima Kazuki  藤田保健衛生大学, その他部局等, 講師 (10442998)

Research Collaborator Hirabayashi Yoshio  国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, シニアチームリーダー (90106435)
Nagatsuka Yasuko  国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, 研究嘱託
Akiyama Hisako  国立研究開発法人理化学研究所, 脳科学総合研究センター・神経膜機能研究チーム, 研究員 (80623462)
Tohda Koji  富山大学, 大学院理工学研究部(工学), 教授 (60212065)
Iwabuchi Kazuhisa  順天堂大学, 環境医学研究所, 教授 (10184897)
Kamiguchi Hiroyuki  国立研究開発法人理化学研究所, 脳科学総合研究センター・神経成長機構研究チーム, チームリーダー (10233933)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsグルコース化脂質 / 固相抽出 / 親水性相互作用クロマトグラフィー / 気相分離 / 脂質メタボローム / 神経新生 / 肥満 / 腎疾患 / イオンモビリティーシステム / 脂質メタボローム解析 / 異性体分離 / アフィテニティクロマトグラフィー / 神経変性疾患
Outline of Final Research Achievements

Mono-glucosylated sphingolipids and glycerophospholipids play important roles in diverse biologicalprocesses and are linked to a variety of pathologies, such as Parkinson disease.The precise identification of the carbohydrate head group of these lipids is complicated by their isobaric nature and by a low abundant amounts in biological samples. Therefore, the challenging researches for monitoring glucosylated lipids is to develop chromatographic method to separate lipids that are not distinguished by regular MS alone. To overcome these obstacles, we have established a novel target lipidomics workflow for efficiently detecting such lipid species. The workflow includes 1) enrichment by affinity chromatography using newly developed benzoboloxole-modified column, 2) separation by zwitterionic hydrophilic interaction chromatography, 3) gas-phase separation by ion-mobility system, and 4) screening assay system by triple quadrupole mass spectrometer.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report

Research Products

(15 results)

All 2017 2016 2015 Other

All Int'l Joint Research Journal Article Presentation Book

  • [Int'l Joint Research] Academia Sinica(台湾)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Separation and analysis of mono-glucosylated lipids in brain and skin by hydrophilic interaction chromatography based on carbohydrate and lipid moiety2016

    • Author(s)
      Nakajima K, Akiyama H, Tanaka K, Kohyama-Koganeya A, Greimel P, Hirabayashi Y.
    • Journal Title

      Journal of Chromatography B

      Volume: 1031 Pages: 146-153

    • DOI
      10.1016/j.jchromb.2016.07.047
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery2016

    • Author(s)
      Kizuka Y, Funayama S, Shogomori H, Nakano M, Nakajima K, Oka R, Kitazume S, Yamaguchi Y, Sano M, Korekane H, Hsu TL, Lee HY, Wong CH, Taniguchi N.
    • Journal Title

      Cell Chem Biol.

      Volume: 23 Pages: 782-792

    • DOI
      10.1016/j.chembiol.2016.06.010
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Aglycon diversity of brain sterylglucosides: structure determination of cholesteryl- and sitosterylglucoside2016

    • Author(s)
      Akiyama H, Nakajima K, Itoh Y, Sayano T, Ohashi Y, Yamaguchi Y, Greimel, P, Hirabayashi Y.
    • Journal Title

      Journal of Lipid Research

      Volume: 57 Pages: 2061-2072

    • DOI
      10.1194/jlr.m071480
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling.2016

    • Author(s)
      Chanmee T, Ontong P, Izumikawa T, Higashide M, Mochizuki N, Chokchaitaweesuk C, Khansai M, Nakajima K, Taniguchi N, Itano N.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Pages: 24105-24120

    • DOI
      10.1074/jbc.m116.751263
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Glucocerebrosidase Deficiency Accelerates the Accumulation of Proteinase K-Resistant α-Synuclein and Aggravates Neurodegeneration in a Drosophila Model of Parkinson’s disease.2015

    • Author(s)
      Suzuki M, Fujikake N, Takeuchi T, Kohyama-Koganeya A, Nakajima K, Hirabayashi Y, Wada K, Nagai Y.
    • Journal Title

      Human Molecular Genetics

      Volume: 24 Pages: 6675-6686

    • DOI
      10.1093/hmg/ddv372
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Elevated O-GlcNAcylation promotes colonic inflammation and tumorigenesis by modulating NF-κB signaling.2015

    • Author(s)
      Yang YR, Kim DH, Seo YK, Park D, Jang HJ, Choi SY, Lee YH, Lee GH, Nakajima K, Taniguchi N, Kim JM, Choi EJ, Moon HY, Kim IS, Choi JH, Lee H, Ryu SH, Cocco L, Suh PG
    • Journal Title

      Oncotarget.

      Volume: 6 Pages: 12529-42

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 腎臓スフィンゴ糖脂質網羅的ワークフローの構築2017

    • Author(s)
      中嶋和紀、高橋和男、伊藤辰将、加藤彰浩、今西進、原田健一、長尾静子、湯澤由紀夫
    • Organizer
      第29会 腎と脂質研究会
    • Place of Presentation
      ホテルグランヴィア広島
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規グライコリピドミクス解析技術による微量糖脂質の神経機能・腎機能の解明2017

    • Author(s)
      中嶋和紀
    • Organizer
      第54回環境医学研究所 第45回順天堂浦安病院研究推進委員会 合同セミナー
    • Place of Presentation
      順天堂大学医学部付属浦安病院
    • Year and Date
      2017-02-10
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 新規脂質メタボローム解析技術を用いた微量グルコース化脂質の神経疾患・腎疾患における役割解明2016

    • Author(s)
      中嶋和紀、秋山央子、長塚靖子、岩渕和久、高橋和男、湯澤由紀夫、平林義雄
    • Organizer
      糖鎖科学中部拠点「第13回若手の力フォーラム」
    • Place of Presentation
      岐阜大学応用生物科学部
    • Year and Date
      2016-11-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] HILIC-ESI-MSによるグルコース化異性体の定性・定量分析とその臨床応用2016

    • Author(s)
      中嶋和紀、秋山央子、長塚靖子、岩渕和久、高橋和男、湯澤由紀夫、平林義雄
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザかるぽーと
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] HILIC-ESI-MSによるグルコース化異性体の定性・定量分析とその臨床応用2016

    • Author(s)
      中嶋和紀、秋山央子、長塚靖子、岩渕和久、高橋和男、湯澤由紀夫、平林義雄
    • Organizer
      第41回 医用マススペクトル会議年会
    • Place of Presentation
      ウィンク愛知
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] HILIC-イオンモビリティ-MS/MSによるホスファチジルグルコシド代謝物の高感度・高選択的定量法2015

    • Author(s)
      中嶋和紀、長塚靖子、中山仁志、岩渕和久、平林義雄
    • Organizer
      第34回 日本糖質学会年会
    • Place of Presentation
      東京大学安田講堂(文京区)
    • Year and Date
      2015-07-31
    • Related Report
      2015 Research-status Report
  • [Presentation] 分離技術を駆使した微量グルコース化脂質の解析アプローチ2015

    • Author(s)
      中嶋和紀
    • Organizer
      第57回 日本脂質生化学会
    • Place of Presentation
      一橋大学一橋講堂(千代田区)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 生体の科学、ホスファチジルグルコシドとそのリゾ体脂質の生物機能2016

    • Author(s)
      長塚靖子、中嶋和紀、平林義雄
    • Publisher
      医学書院
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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