Elucidation of regulation of osteogenesis and chondrogenesis by Hippo pathway

• Project/Area Number: 15K19026
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: Goto Hiroki 九州大学, 生体防御医学研究所, 助教 (20734495)
• Research Collaborator: NISHIO Miki
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Hippo経路 / シグナル伝達 / Ｈｉｐｐｏ経路

Outline of Final Research Achievements

Osteoblast-specific Mob1a/1b double homozygous mutant mice showed osteosclerosis with the growth of osteoblasts. Chondrocyte-specific Mob1a/1b double homozygous mutant mice showed short-limb skeletal dysplasia with the reduction of growth plate length. Genes related with extracellular matrix in chondrocyte-specific Mob1 deleted cells were downregulated in vitro. These results indicate that Hippo pathway plays a critical role in the regulation of osteogenesis and chondrogenesis.

Research Products

• [Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene (2017)
  • Author(s): Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
  • Journal Title: Oncogene Volume: 印刷中 Issue: 29 Pages: 4201-4211
  • DOI: 10.1038/onc.2017.58
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hippo vs. Crab: tissue-specific functions of the mammalian Hippo pathway (2017)
  • Author(s): M. Nishio, T. Maehama, H. Goto, K. Nakatani, W. Kato, H. Omori, Y. Miyachi, H. Togashi, Y. Shimono and A. Suzuki
  • Journal Title: Genes to Cells Volume: 22 Issue: 1 Pages: 6-31
  • DOI: 10.1111/gtc.12461
  • Peer Reviewed / Open Access
• [Journal Article] Targeting the Hippo signalling pathway for cancer treatment (2016)
  • Author(s): K. Nakatani, T. Maehama, M. Nishio, H. Goto, W. Kato, H. Omori, Y. Miyachi, H. Togashi, Y. Shimono and A. Suzuki
  • Journal Title: J. Biochem. Volume: 161 Pages: 237-244
  • DOI: 10.1093/jb/mvw074
  • NAID: 40021162008
  • Peer Reviewed / Open Access
• [Journal Article] Dysregulated YAP1/TAZ and TGF signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice (2015)
  • Author(s): Nishio, M., Sugimachi, K., Goto, H., Wang, J., Morikawa, T., Miyachi, Y., Takano, Y., Hikasa, H., Itoh, T., Suzuki, S.O., Kurihara, H., Aishima, S., Leask, A., Sasaki, T., Nakano, T., Nishina, H., Nishikawa, Y., Sekido, Y., Nakao, K., Shin-ya, K., Mimori, K. and Suzuki, A.
  • Journal Title: PNAS Volume: 113 Issue: 1 Pages: 71-80
  • DOI: 10.1073/pnas.1517188113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Regulation of chondrogenesis and osteogenesis by Hippo pathway (2015)
  • Author(s): Hiroki Goto, Miki Nishio, Akira Suzuki
  • Organizer: The 25th Hot Spring Harbor International Symposium, Cutting Edge of Technical Innovations in Structural and Systems Biology 2015
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-13