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Functional analysis of gangliosides in invasion front in early stage glioma

Research Project

Project/Area Number 15K19073
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionChubu University

Principal Investigator

OHKAWA Yuki  中部大学, 生命健康科学部, 研究員 (40723896)

Co-Investigator(Renkei-kenkyūsha) FURUKAWA Koichi  中部大学, 生命健康科学部, 教授 (80211530)
MOMOTA Hiroyuki  東京大学, 医科学研究所, 講師 (60469971)
NATSUME Atsushi  名古屋大学, 医学系研究科, 准教授 (30362255)
WAKABAYASHI Toshihiko  名古屋大学, 医学系研究科, 教授 (50220835)
Research Collaborator KATO Akira  名古屋大学, 医学系研究科, 研究員
HASHIMOTO Noboru  徳島大学, 医歯薬学研究部, 助教
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsガングリオシド / グリオーマ / マウスモデル / EMARS
Outline of Final Research Achievements

Acidic glycosphingolipids, ganglioside GD3 and GD2 are expressed in gliomas strongly, while expression levels of them in normal brain tissues are minimal. In this study, we tried to clarify functions of GD3 and GD2 in invasion front in early stage of gliomas using gene-engineered murine glioma model. Results indicated that GD3 and GD2 enhanced invasion activity by inducing over-expression of matrixmetalloproteinase, Mmp9 via activation of a transcriptional factor Ap2α. These data suggest advantages to establish GD3- or GD2-targeted therapies for glioma patients.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2016 2015

All Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 10 results,  Open Access: 6 results,  Acknowledgement Compliant: 7 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Expression of B4GALNT1, an essential glycosyltransferase for the synthesis of complex gangliosides, suppresses BACE1 degradation and modulates APP processing2016

    • Author(s)
      Yamaguchi T, Yamauchi Y, Furukawa K, Ohmi Y, Ohkawa Y, Zhang Q, Okajima T, Furukawa K
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 34505-34505

    • DOI

      10.1038/srep34505

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Increased a-series gangliosides positively regulate leptin/Ob receptor-mediated signals in hypothalamus of GD3 synthase-deficient mice.2016

    • Author(s)
      Ji S, Tokizane K, Ohkawa Y, Ohmi Y, Banno R, Okajima T, Kiyama H, Furukawa K and Furukawa K.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 479(3) Issue: 3 Pages: 453-460

    • DOI

      10.1016/j.bbrc.2016.09.077

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes.2016

    • Author(s)
      Bhuiyan RH, Kondo Y, Yamaguchi T, Tokuda N, Ohkawa Y, Hashimoto N, Ohmi Y, Yamauchi Y, Furukawa K, Okajima T and Furukawa K.
    • Journal Title

      Glycobiology

      Volume: 26(9) Pages: 984-998

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A therapeutic trail of human melanoma with combined small interfering RNAs targeting adaptor molecules p130Cas and paxillin activated under expression of ganglioside GD3.2016

    • Author(s)
      Makino Y, Hamamura K, Takei Y, Bhuiyan RH, Ohkawa Y, Ohmi Y, Nakashima H, Furukawa K, Furukawa K.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: in press Issue: 8 Pages: 1753-1763

    • DOI

      10.1016/j.bbagen.2016.04.005

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sialylation converts arthritogenic IgG into inhibitors of collagen-induced arthritis.2016

    • Author(s)
      Ohmi Y, Ise W, Harazono A, Takakura D, Fukuyama H, Baba Y, Narazaki M, Shoda H, Takahashi N, Ohkawa Y, Ji S, Sugiyama F, Fujio K, Kumanogoh A, Yamamoto K, Kawasaki N, Kurosaki T, Takahashi Y, Furukawa K.
    • Journal Title

      Nature Communications

      Volume: in press Issue: 1 Pages: 11205-11205

    • DOI

      10.1038/ncomms11205

    • NAID

      120007135549

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes.2016

    • Author(s)
      Bhuiyan RH, Kondo Y, Yamaguchi T, Tokuda N, Ohkawa Y, Hashimoto N, Ohmi Y, Yamauchi Y, Furukawa K, Okajima T, Furukawa K.
    • Journal Title

      Glycobiology

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ganglioside GD3 enhances invasiveness via Yes activation by forming a complex of GD3/PDGFRα/Yes in gliomas2015

    • Author(s)
      Yuki Ohkawa, Hiroyuki Momota, Akira Kato, Noboru Hashimoto, Yuhsuke Tsuda, Norihiro Kotani, Koichi Honke, Akio Suzumura, Keiko Furukawa, Yuhsuke Ohmi, Atsushi Natsume, Toshihiko Wakabayashi and *Furukawa K
    • Journal Title

      J. Biol. Chem.

      Volume: 290 Issue: 32 Pages: 16043-16058

    • DOI

      10.1074/jbc.m115.708834

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase.2015

    • Author(s)
      Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
    • Journal Title

      J Biol Chem.

      Volume: 290 Issue: 26 Pages: 16043-16058

    • DOI

      10.1074/jbc.m114.635755

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulatory function of glycosphingolipids in the inflammation and degeneration.2015

    • Author(s)
      Furukawa K., Ohmi Y., Kondo Y., Ohkawa Y., Tajima O. and Furukawa K
    • Journal Title

      Arch Biochem Biophys

      Volume: 571 Pages: 58-65

    • DOI

      10.1016/j.abb.2015.02.007

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] b-series gangliosides crucially regulate leptin secretion in adipose tissues.2015

    • Author(s)
      Ji S., Ohkawa Y., Tokizane K., Ohmi Y., Banno R., Furukawa K., Kiyama H. and Furukawa K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 459 Issue: 2 Pages: 189-195

    • DOI

      10.1016/j.bbrc.2015.01.143

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Identification of GD3-regulated genes in GD3-expressing gliomas.2016

    • Author(s)
      Ohkawa Y, Noda S, Momota H, Kato A, Chou B, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T and Furukawa K.
    • Organizer
      第75回 日本癌学会学術総会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] グリオーマにおいてガングリオシドGD3はPDGFRαの発現を誘導する2016

    • Author(s)
      大川祐樹、百田洋之、加藤彰、橋本登、古川圭子、大海雄介、夏目敦至、若林俊彦、古川鋼一
    • Organizer
      第35回 日本糖質学会年会
    • Place of Presentation
      高知県高知市 文化プラザ かるぽーと
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] Loss of b-series gangliosides attenuates glioma development and expansion.2016

    • Author(s)
      Ohkawa Y, Momota H, Kato A, Esaki N, Chou B, Takano M, Bhuiyan RH, Furukawa K, Natsume A, Wakabayashi T, Furukawa K.
    • Organizer
      The 3rd International Symposium on Glycol-Neuroscience
    • Place of Presentation
      淡路夢舞台国際会議場、兵庫県淡路市
    • Year and Date
      2016-01-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expression of gangliosides GD3 promotes development and expansion of gliomas in glioma model mice.2015

    • Author(s)
      Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Esaki N, Furukawa K, Natsume A, Wakabayashi T, Furukawa K.
    • Organizer
      第74回 日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場、愛知県名古屋市
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] Ganglioside GD3 promotes development and invasion of gliomas.2015

    • Author(s)
      Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
    • Organizer
      第27回 日本糖質学会
    • Place of Presentation
      東京大学安田講堂、東京都文京区
    • Year and Date
      2015-07-31
    • Related Report
      2015 Research-status Report
  • [Presentation] Analysis of mechanisms for induction of GD3 in astrocytes and glioma cells.2015

    • Author(s)
      Ohkawa Y, Ohkochi T, Esaki N, Takano M, Bhuiyan RH, Chou B, Ohmi Y, Furukawa K, Furukawa K.
    • Organizer
      平成27年度 神経糖鎖生物学 夏の班会議
    • Place of Presentation
      とりぎん文化会館、鳥取県鳥取市
    • Year and Date
      2015-06-25
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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