| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Vibrio alginolyticus posses three CsrB family regulatory RNA. In this study, we investigated the mechanism of these RNAs expression system in transcriptional and post-transcriptional level. First, we checked stability among these RNAs using rifampicin RNA-chase assay. As a result, no any difference of RNA stability in three RNAs was shown. Next, we analyzed the possibility that ORF regions affect transcriptional activity. However, reporter assay indicated that the no difference of the activity was shown in ORF containing promoter. These resultu indicated that unknown mechanism produce the non-redundancy among three CsrB family regulatory RNAs.
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