Mechanistic analysis of viral replication using anti-DENV compound
Project/Area Number |
15K19109
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Virology
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
HISHIKI Takayuki 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (30535761)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | デングウイルス / 複製機構 / 抗ウイルス化合物 / ケミカルバイオロジー / 抗ウイルス剤 / 抗ウイルス |
Outline of Final Research Achievements |
Dengue virus (DENV) infectious disease is a major public health problem worldwide; however, specific antiviral drugs against this infection are not available. To identify novel anti-DENV compounds, we screened pharmacologically active compounds using focus reduction assay. We identified some compounds that have potent anti-DENV activity and low cytotoxicity. Time-of-drug-addition and time-of-drug-elimination assays revealed that compound inhibits translation and/or replication steps in the DENV life cycle. A subgenomic replicon system was used to verify that compound restricts RNA replication step. Furthermore, analysis of compound-resistant clone suggested that compound targets viral nonstructural protein.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Novel antiviral activity of bromocriptine against dengue virus replication.2016
Author(s)
Kato Fumihiro, Ishida Yuki, Oishi Shinya, Fujii Nobutaka, Watanabe Satoru, Vasudevan Subhash, Tajima Shigeru, Takasaki Tomohiko, Suzuki Yoichi, Ichiyama Koji, Yamamoto Naoki, Yoshii Kentaro, Takashima Ikuo, Kobayashi Takeshi, Miura Tomoyuki, Igarashi Tatsuhiko, Hishiki Takayuki.
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Journal Title
Antiviral Res.
Volume: 12
Pages: 141-7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Generation of a neutralization-resistant CCR5 tropic SHIV-MK38 molecular clone, a derivative of SHIV-89.6.2016
Author(s)
Ishida Yuki, Yoneda Mai, Otsuki Hiroyuki, Watanabe Yuji, Kato Fumihiro, Matuura Kanako, Kikukawa Minako, Matsushita Shuzo, Hishiki Takayuki, Igarashi Tatsuhiko, Miura Tomoyuki.
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Journal Title
J Gen Virol
Volume: 97(5)
Pages: 1249-60
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication.2016
Author(s)
Suzuki Youichi, Chin Weixin, Han Qien, Ichiyama Koji, Lee Ching, Eyo Zhin, Ebina Hirotaka, Takahashi Hirotaka, Takahashi Chikako, Tan Beng, Hishiki Takayuki, Ohba Kenji, Matsuyama Toshifumi, Koyanagi Yoshio, Tan Yee, Sawasaki Tatsuya, Chu Justin, Vasudevan Subhash, Sano Kouichi, Yamamoto Naoki.
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Journal Title
PLoS Pathog
Volume: 6;12(1)
Pages: e1005357
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Murine Herc6 plays a critical role in protein ISGylation in vivo and has an ISGylation independent function in seminal vesicles.2015
Author(s)
Arimoto Keiichiro, Hishiki Takayuki, Kiyonari Hiroshi, Abe Takaya, Cheng Chuyi, Yan Ming, Fan Jun, Futakuchi Mitsuru, Tsuda Hiroyuki, Murakami Yoshiki, Suzuki Hideyuki, Zhang Dong, Shimotohno Kunitada.
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Journal Title
J Interferon Cytokine Res
Volume: 35(5)
Pages: 351-8
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Bromocriptine is a novel inhibitor of dengue virus replication2016
Author(s)
Takayuki Hishiki, Fumihiro Kato, Yuki Ishida, Shinya Oishi, Satoru Watanabe, Subhash G. Vasudevan, Shigeru Tajima, Tomohiko Takasaki, Tomoyuki Miura, and Tatsuhiko Igarashi
Organizer
4th Antivirals Congress
Place of Presentation
Barcelona (Spain)
Year and Date
2016-09-18 – 2016-09-21
Related Report
Int'l Joint Research
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[Book] Hepatitis C Virus I2016
Author(s)
Hishiki Takayuki, Shimizu Yuko, Ujino Saneyuki, Nishitsuji Hironori, Shimotohno Kunitada.
Total Pages
18
Publisher
Springer book
Related Report
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