Regulatory mechanisms of autoimmunity by LAG-3 expressing cells

• Project/Area Number: 15K19132
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: The University of Tokushima
• Principal Investigator: OKAZAKI Il-mi 徳島大学, 先端酵素学研究所(プロテオ), 准教授 (50452339)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 抑制性免疫補助受容体 / 単一細胞発現解析 / 自己免疫疾患

Outline of Final Research Achievements

LAG-3 is an immuno-inhibitory receptor that plays a critical role in the prevention of autoimmunity. To understand cell population(s) and their role(s) on which LAG-3 functions in the regulation of autoimmunity, we analyzed LAG-3 expressing CD4 T cells by single-cell gene expression analysis. We found that LAG-3 expressing cells were clustered into distinct cell populations and identified unique cell populations that may function in the suppression of autoimmunity.

Research Products

• [Journal Article] 抑制性免疫補助受容体を標的としたがん免疫療法の基礎研究 (2016)
  • Author(s): 岡崎 拓、岡崎一美
  • Journal Title: 医学のあゆみ Volume: 256 Pages: 15861-15864
• [Presentation] Characterization of target genes of inhibitory co-receptor PD-1 by CAGE (2017)
  • Author(s): Shimizu K, Sugiura D, Maruhashi T, Okazaki IM, Okazaki T
  • Organizer: The 11th International Symposium of the institute network "Frontiers in Biomedical Sciences"
  • Place of Presentation: 徳島大学（徳島県・徳島市）
  • Year and Date: 2017-01-26
  • Int'l Joint Research
• [Presentation] Glucocorticoids augment the expression and inhibitory function of PD-1 (2017)
  • Author(s): Maeda N, Maruhashi T, Shimizu K, Okazaki IM, Okazaki T
  • Organizer: The 11th International Symposium of the institute network "Frontiers in Biomedical Sciences"
  • Place of Presentation: 徳島大学（徳島県・徳島市）
  • Year and Date: 2017-01-26
  • Int'l Joint Research
• [Presentation] Characterization of target genes of inhibitory co-receptor PD-1 by CAGE (2016)
  • Author(s): SHIMIZU Kenji, SUGIURA Daisuke, MARUHASHI Takumi, OKAZAKI Il-mi, OKAZAKI Taku
  • Organizer: The 45th annual meeting of the JSI
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Glucocorticoids augment the expression and inhibitory function of PD-1 (2016)
  • Author(s): MAEDA Natsumi, MARUHASHI Takumi, SHIMIZU Kenji, OKAZAKI Il-mi, OKAZAKI Taku
  • Organizer: The 45th annual meeting of the JSI
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県・宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] マイノリティ細胞の同定と解析による自己およびがんに対する免疫制御機構の解明 (2016)
  • Author(s): 岡崎一美、清水謙次、岡崎 拓
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Establishment and maintenance of immune tolerance by immuno-inhibitory receptors (2016)
  • Author(s): Maruhashi T, Okazaki IM, Sugiura D, Okazaki T
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
  • Invited
• [Presentation] Characterization of target genes of inhibitory co-receptor PD-1 by CAGE (2016)
  • Author(s): Shimizu K, Sugiura D, Maruhashi T, Okazaki IM, Okazaki T
  • Organizer: The 26th Hot Spring Harbor International Symposium
  • Place of Presentation: 九州大学（福岡県・福岡市）
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] Phenotypic characterization of LAG-3 expressing cells (2016)
  • Author(s): Okazaki IM, Okazaki T
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Inhibition of T cell activation by human LAG-3 (2016)
  • Author(s): Kajihara T, Sugiura D, Maruhashi T, Okazaki IM, Okazaki T
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Context-dependent inhibition of antigen-specific T cell activation by LAG-3 (2016)
  • Author(s): Maruhashi T, Okazaki IM, Sugiura D, Okazaki T
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne (Australia)
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] The strength of inhibition by LAG-3 depends on properties of APCs. (2015)
  • Author(s): MARUHASHI Takumi, OKAZAKI Il-mi, SUGIURA Daisuke, KAJIHARA Takeo, OKAZAKI Taku
  • Organizer: The 44th annual meeting of the JSI
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Comparison of human and mouse LAG-3. (2015)
  • Author(s): KAJIHARA Takeo, SUGIURA Daisuke, MIZUNO Reina, RANAWAKAGE Chamila Deshani, MAEDA Natsumi, SHIMIZU Kenji, OKAZAKi Il-mi, OKAZAKI Taku
  • Organizer: The 44th annual meeting of the JSI
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2015-11-18