Imaging mass spectrometry of antibody drugs
| Project/Area Number |
15K19170
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied pharmacology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 質量分析 / イメージング / 抗体医薬 / イメージング質量分析 / 組織上消化 / 可変領域 |
|---|
| Outline of Final Research Achievements |
The main purpose of this research proposal is to visualize the distribution of an antibody drug, Herceptin. As the first step, the target protein was digested with trypsin and specific peptides were detected. However, in the mixture of human plasma and Herceptin, many peptides peaks derived from IgG were detected with high intensity. On the tissue surface, this situation was not improved. Although many peptides derived from abundant proteins such as cytoskeleton were detected, the target peptides were not detected due to ion suppression. Through this research, it is revealed that proposed methodology could not solve the problems to visualize antibody drugs. I consider completely different approaches are essential to achieve the purpose.
|
Report
(3 results)
Research Products
(2 results)
