Elucidation of the role of Zinc transporter in intestinal stem cell maintenance.

• Project/Area Number: 15K19319
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: University of Toyama
• Principal Investigator: Ohashi Wakana 富山大学, 大学院医学薬学研究部(医学), 助教 (50381596)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腸管上皮細胞 / 亜鉛トランスポーター / 腸管上皮幹細胞 / 小胞体ストレス / 亜鉛 / 腸管恒常性

Outline of Final Research Achievements

Zinc transporters play a central role in the maintenance of zinc homeostasis. Emerging evidence indicates important roles of zinc transporters in the regulation of various tissue functions. In this study, using the intestinal-epithelial specific or Lgr5-positive stem cell-specific ZIP7 deletion mice, we show that ZIP7 contribute to the maintenance of intestinal epithelial homeostasis by resolving ER stress in transit amplifying cells and intestinal stem cells.

Research Products

• [Int'l Joint Research] Institut Curie(France)
• [Journal Article] Emerging role of zinc homeostasis by zinc transporter ZIP7 in intestinal homeostatic self-renewal (2017)
  • Author(s): Ohashi W, Hase K, Fukada T.
  • Journal Title: Inflammation & Cell signaling Volume: ４
  • DOI: 10.14800/ics.1509
  • Open Access / Acknowledgement Compliant
• [Journal Article] Zinc transporter SLC39A7/ZIP7 promotes intestinal epithelial self-renewal by resolving ER stress. (2016)
  • Author(s): Ohashi, W., Kimura, S., Iwanaga, T., Furusawa, Y., Irie, T., Izumi, H., Watanabe, T., Hijikata, A., Hara, T., Ohara, O., Koseki, H., Sato, T., Robine, S., Mori, H., Hattori, Y., Watarai, H., Mishima, K., Ohno, H., Hase, K., Fukada, T.
  • Journal Title: PLoS Genetics Volume: 12 Issue: 10 Pages: e1006349-e1006349
  • DOI: 10.1371/journal.pgen.1006349
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Critical role of endogenous histamine in promoting end-organ tissue injury in sepsis. (2016)
  • Author(s): Hattori M, Yamazaki M, Ohashi W, Tanaka S, Hattori K, Todoroki K, Fujimori T, Ohtsu H, Matsuda N, Hattori Y.
  • Journal Title: Intensive Care Med Exp. Volume: ４ Issue: 1 Pages: 36-44
  • DOI: 10.1186/s40635-016-0109-y
  • Peer Reviewed / Open Access
• [Journal Article] 腸管恒常性における亜鉛トランスポーターの役割 “The role of Zinc transporter in the maintenance of intestinal epithelial homeostasis” (2016)
  • Author(s): 大橋若奈
  • Journal Title: 亜鉛栄養治療 Volume: 7 Pages: 22-34
• [Journal Article] Control of macrophage dynamics as a potential therapeutic approach for clinical disorders involving chronic inflammation. (2015)
  • Author(s): Wakana Ohashi, Kohshi Hattori, Yuichi Hattori
  • Journal Title: J Pharmacol Exp Ther Volume: 354巻，3号 Issue: 3 Pages: 240-250
  • DOI: 10.1124/jpet.115.225540
  • Peer Reviewed
• [Presentation] 亜鉛トランスポーターによる小胞体ストレスと腸管上皮恒常性の制御機構 (2016)
  • Author(s): Wakana Ohashi, Koji Hase, Toshiyuki Fukada
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] 腸管恒常性における亜鉛トランスポーターの役割 (2016)
  • Author(s): 大橋若奈
  • Organizer: 第13回 亜鉛栄養治療研究会
  • Place of Presentation: 大阪新丸ビル
  • Year and Date: 2016-08-05
  • Invited
• [Presentation] 腸管上皮の自己複製機構における亜鉛トランスポーターの役割 (2016)
  • Author(s): 大橋若奈、長谷耕二、深田俊幸
  • Organizer: 第93回日本生理学会大会 シンポジウム14
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2016-03-22