pathogenesis of bird-related hypersensitivity pneumonitis

• Project/Area Number: 15K19415
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: FURUSAWA Haruhiko 東京医科歯科大学, 医学部附属病院, 助教 (40632154)
• Co-Investigator(Renkei-kenkyūsha): SHIRAI Tsuyoshi 東京医科歯科大学, 医学部附属病院, 助教 (00796617)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 間質性肺炎 / 過敏性肺炎 / IGLL / 鳥関連過敏性肺炎 / 慢性過敏性肺炎 / 質量分析 / ＩＧＬＬ

Outline of Final Research Achievements

Bird-related hypersensitivity pneumonitis (BRHP) is an interstitial pneumonia mainly caused by avian antigen, however the causative protein was still unidentified. In the present study, we performed an immunoblot analysis using pigeon dropping extract with sera from patients with BRHP and control. Patient-specific bands were analyzed by mass spectrometry and we identified the causative antigen as Immunoglobulin lambda-like polypeptide (IGLL-1). Then we evaluated the antigenicity of IGLL-1 using recombinant protein by ELISA and peripheral blood mononuclear cells (PBMCs) proliferative assay. BRHP patients exhibited higher levels of serum IgG antibody against the recombinant IGLL-1 compared to the control subjects, as well as a stronger PBMCs proliferative response to the protein.

Research Products

• [Journal Article] Efficacy of pirfenidone in chronic hypersensitivity pneumonitis (2018)
  • Author(s): Sho Shibata, Haruhiko Furusawa, Naohiko Inase
  • Journal Title: Sarcoidosis, vasculitis and diffuse lung disease Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Direct hemoperfusion with polymyxin B-immobilized fibre treatment for acute exacerbation of interstitial pneumonia (2017)
  • Author(s): Furusawa Haruhiko、Sugiura Makiko、Mitaka Chieko、Inase Naohiko
  • Journal Title: Respirology Volume: 22 Issue: 7 Pages: 1357-1362
  • DOI: 10.1111/resp.13054
  • Peer Reviewed
• [Journal Article] Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping. (2017)
  • Author(s): Shirai T, Furusawa H, Furukawa A, Ishige Y, Uchida K, Miyazaki Y, Eishi Y, Inase N.
  • Journal Title: Respiratory Research Volume: 18 Issue: 1 Pages: 65-65
  • DOI: 10.1186/s12931-017-0555-4
  • Peer Reviewed
• [Journal Article] Chronic Hypersensitivity Pneumonitis With a Usual Interstitial Pneumonia-Like Pattern : Correlation Between Histopathologic and Clinical Findings (2016)
  • Author(s): Sahoko Chiba, Kimitake Tsuchiya, Takumi Akashi, Masahiro Ishizuka, Tsukasa Okamoto, Haruhiko Furusawa, Tomoya Tateishi, Mitsuhiro Kishino, Yasunari Miyazaki, Ukihide Tateishi, Tamiko Takemura, Naohiko Inase
  • Journal Title: chest Volume: 149 Issue: 6 Pages: 1473-1481
  • DOI: 10.1016/j.chest.2015.12.030
  • Peer Reviewed
• [Journal Article] サルコイドーシスとその周辺疾患一肺肉芽腫性疾患一 (2016)
  • Author(s): 宮崎泰成，古澤春彦，須原宏造，稲瀬直彦
  • Journal Title: 日本サルコイドーシス/肉芽腫性疾患学会雑誌 Volume: 36 Pages: 27-30
  • Peer Reviewed
• [Presentation] Bronchoalveolar lavage in chronic hypersensitiity pneumonitis and IPF. (2017)
  • Author(s): Furusawa Haruhiko, Saito Hiroaki, Sema Manabu, Tateishi tomoya, Miyazaki Yasunari, Inase Naohiko
  • Organizer: American thoracic society
  • Int'l Joint Research
• [Presentation] サルコイドーシスの抗菌療法‐医師主導臨床試験について‐ (2017)
  • Author(s): 古澤春彦
  • Organizer: 日本サルコイドーシス/肉芽腫性疾患学会
• [Presentation] Bronchoalveolar Lavage in Chronic Hypersensitivity Pneumonitis and IPF (2017)
  • Author(s): Haruhiko Furusawa, Hiroaki Saito, Manabu Sema, Tomoya Tateishi, Yasunari Miyazaki, Naohiko Inase
  • Organizer: ATS 2017
  • Place of Presentation: ワシントンDC(米国）
  • Int'l Joint Research
• [Presentation] The Usefulness of Direct Hemoperfusion with Polymyxin B-Immobilized Fiber (PMX-DHP) Treatment on Acute Exacerbation of Interstitial Pneumonitis (2016)
  • Author(s): Haruhiko Furusawa, Makiko Sugiura, Yasunari Miyazaki, Naohiko Inase
  • Organizer: ATS2016
  • Place of Presentation: サンフランシスコ（米国）
  • Int'l Joint Research
• [Presentation] 当院における過敏性肺炎の気管支肺胞洗浄液の検討 (2016)
  • Author(s): 古澤 春彦・石塚 聖洋・立石 知也・土屋 公威・藤江 俊秀・玉岡 明洋・坂下 博之・宮崎 泰成・稲瀬 直彦
  • Organizer: 第39回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県、名古屋市）
• [Presentation] 鳥関連過敏性肺炎の責任抗原の同定 (2015)
  • Author(s): 白井剛
  • Organizer: びまん性肺疾患に対するエビデンスを構築する新規戦略的研究班 平成27年度第二回班会議総会
  • Place of Presentation: 東邦大学医療センター大森病院（東京都大田区）
  • Year and Date: 2015-12-12
• [Book] 気管支肺胞洗浄（BAL）法の手引き (2017)
  • Author(s): 古澤春彦、稲瀬直彦
  • Total Pages: 218
  • Publisher: 克誠堂
  • ISBN: 9784771904897