| Project/Area Number |
15K19415
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIRAI Tsuyoshi 東京医科歯科大学, 医学部附属病院, 助教 (00796617)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 間質性肺炎 / 過敏性肺炎 / IGLL / 鳥関連過敏性肺炎 / 慢性過敏性肺炎 / 質量分析 / ＩＧＬＬ |
|---|
| Outline of Final Research Achievements |
Bird-related hypersensitivity pneumonitis (BRHP) is an interstitial pneumonia mainly caused by avian antigen, however the causative protein was still unidentified. In the present study, we performed an immunoblot analysis using pigeon dropping extract with sera from patients with BRHP and control. Patient-specific bands were analyzed by mass spectrometry and we identified the causative antigen as Immunoglobulin lambda-like polypeptide (IGLL-1). Then we evaluated the antigenicity of IGLL-1 using recombinant protein by ELISA and peripheral blood mononuclear cells (PBMCs) proliferative assay. BRHP patients exhibited higher levels of serum IgG antibody against the recombinant IGLL-1 compared to the control subjects, as well as a stronger PBMCs proliferative response to the protein.
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