The role of cancer stem cell-like sphere forming cells in multi-organ metastasis of lung cancer

• Project/Area Number: 15K19420
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: The University of Tokushima
• Principal Investigator: OGINO Hirokazu 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (20745294)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肝転移 / 癌幹細胞 / 肺癌

Outline of Final Research Achievements

In this study, we investigated the roles of cancer stem cells (CSCs) in forming multiple organ metastasis in mice. First, we isolated the CSC-like populations from two human lung cancer cell lines by culturing in low-attachment and low-nutrient condition, and confirmed these populations showed higher gene expressions of stem cell markers. Next, we found the number of liver metastasis was increased in mice inoculated with CSC-like populations in multiple organ metastasis model. By analyzing the gene expressions of CSC-like populations and parental cells, several candidate genes were picked-up that might regulate liver metastasis. In this study, we focused on gene X, and confirmed that the specific inhibition of gene X of CSC-like populations by RNAi resulted in the suppression of liver metastasis in mice. These results indicate that gene X might regulate the production of liver specific metastasis, therefore it might be a good therapeutic target to control the liver metastasis.

Research Products

• [Presentation] 小細胞肺癌における肝転移の位置づけとがん幹細胞様細胞の役割 (2016)
  • Author(s): 後東久嗣, 荻野広和, 三橋惇志, 西岡安彦
  • Organizer: 第25回日本がん転移学会学術集会
  • Place of Presentation: 米子コンベンションセンター（鳥取県・米子市）
• [Presentation] Sphere 形成癌幹細胞様分画のヒト肺癌多臓器転移モデルマウスにおける役割についての解析. (2015)
  • Author(s): 荻野広和, 三橋惇志, 後東久嗣, 西條敦郎, 佐藤正大, 坂口暁, 手塚敏史, 倉本卓哉, 田畑祥, 上原久典, 埴淵昌毅, 西岡安彦.
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋市）
  • Year and Date: 2015-10-10
• [Presentation] Sphere 形成癌幹細胞様分画の肺癌多臓器転移における役割についての解析 (2015)
  • Author(s): 荻野広和, 後東久嗣, 三橋惇志, 西條敦郎, 佐藤正大, 柿内聡司, 埴淵昌毅, 上原久典, 西岡安彦.
  • Organizer: 第24回日本がん転移学会学術集会
  • Place of Presentation: シティプラザ大阪（大阪府・大阪市）
  • Year and Date: 2015-07-23
• [Presentation] Characterization of Sphere-Forming Stem-Like Population of Lung Cancer Cell Line in Multi-Organ Metastasis Model. (2015)
  • Author(s): Hirokazu Ogino, Atsushi Mitsuhashi, Hisatsugu Goto, Atsuro Saijo, Takuya Kuramoto, Sho Tabata, Hisanori Uehara, Soji Kakiuchi, Masaki Hanibuchi, Yasuhiko Nishioka.
  • Organizer: ATS 2015 International Conference.
  • Place of Presentation: Denver, CO (USA)
  • Year and Date: 2015-05-19
  • Int'l Joint Research