Identification of new deposited nephropathy and elucidation of pathological target

• Project/Area Number: 15K19452
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Takeda Naoko 滋賀医科大学, 医学部, 特任助教 (20737655)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 沈着腎症 / 蛋白質同定

Outline of Final Research Achievements

Glomerular deposits of deposited nephropathy that can not be classified as known diseases experienced at our hospital were analyzed. The glomerulus was separated by laser microdissection and analyzed by LC-MS/MS to identify the etiological protein. Analysis was performed using residual renal biopsy specimens that were used for diagnosis, and the total area of 1 mm2, which is about 20% of the usual volume, was collected. As a result of the analysis, 234 constituent proteins could be identified. We investigated pathogenic proteins using minimal change nephrotic syndrome without deposits and renal amyloidosis specimens with obvious deposits as controls. We are still studying now, and in the future we plan to produce antibodies to candidate proteins, stain with them, and identify deposits.