Investigation of novel differentiation of megakaryocyte and its application in the mouse model

• Project/Area Number: 15K19542
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: University of Tsukuba
• Principal Investigator: KURITA Naoki 筑波大学, 医学医療系, 講師 (30555561)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 巨核球前駆細胞 / 臍帯血骨髄内移植

Outline of Final Research Achievements

Gene expression at single cell level was analyzed with megakaryocytic progenitors extracted from human cord blood. The expression of megakaryocyte-related genes such as cMpl, GPIba, and vWF was observed, which is similar to the expression of the megakaryocytic progenitor that we previously found in mice.
Intrabone-marrow transplantation of cord blood was performed with 15 adult cases with hematological malignancies. Platelet recovery was seen in 13 cases, which was superior to that of intravenous cord-blood transplantation. On-site hematopoiesis was enhanced after intrabone-marrow transplantation. These results suggest that megakaryocytic progenitors of cord blood which engraft at the local site contribute to platelet production after transplantation.

Research Products

• [Journal Article] The road map for megakaryopoietic lineage from hematopoietic stem/progenitor cells (2017)
  • Author(s): Nishikii H, Kurita N, Chiba S
  • Journal Title: Stem Cells Translational Medicine Volume: in press
  • NAID: 120007128846
  • Peer Reviewed
• [Journal Article] A phase I/II trial of intrabone marrow cord blood transplantation and comparison of the hematological recovery with the Japanese nationwide database (2017)
  • Author(s): Kurita N, Gosho M, Yokoyama Y, Kato T, Obara N, Sakata-Yanagimoto M, Hasegawa Y, Uchida N, Takahashi S, Kouzai Y, Atsuta Y, Kurata M, Ichinohe T, Chiba S
  • Journal Title: Bone Marrow Transplantation Volume: 52 Issue: 4 Pages: 574-579
  • DOI: 10.1038/bmt.2016.319
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Comparison of rTM and conventional therapy for DIC in cases with hematological malignancies (2016)
  • Author(s): Naoki Kurita, Yasuhisa Yokoyama, Takayasu Kato, Mamiko Sakata-Yanagimoto, Naoshi Obara, Yuichi Hasegawa, Shigeru Chiba
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
• [Presentation] Recombinant thrombomodulin ameliorates hematological malignancy-induced disseminated intravascular coagulation more promptly than conventional therapy without causing severe hemorrhagic events (2016)
  • Author(s): Naoki Kurita, Yasuhisa Yokoyama, Hideharu Muto, Takayasu Kato, Mamiko Sakata-Yanagimoto, Naoshi Obara, Yuichi Hasegawa, Shigeru Chiba
  • Organizer: 21st congress of European Hematology Association
  • Place of Presentation: Bella center (Copenhagen, Denmark)
  • Year and Date: 2016-06-09
  • Int'l Joint Research
• [Presentation] Improved Platelet Recovery after Intra-Bone Marrow Cord Blood Transplantation: a Result of Phase I/II Trial and a Comparison with Japanese Nation-Wide Transplant Database (2016)
  • Author(s): Naoki Kurita, Masahiko Gosho, Yasuhisa Yokoyama, Hideharu Muto, Takayasu Kato, Mamiko Sakata-Yanagimoto, Naoshi Obara, Yuichi Hasegawa, Yoshiko Atsuta, Tatsuo Ichinohe, Francesco Frassoni, Shigeru Chiba
  • Organizer: The 7th JSH International Symposium 2016
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2016-05-13
  • Int'l Joint Research