The mechanism of aberrant glycosylation in rheumatoid arthritis

• Project/Area Number: 15K19572
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Kanazawa University
• Principal Investigator: Ito Kiyoaki 金沢大学, 附属病院, 医員 (10467110)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 関節リウマチ / IgG / 糖鎖

Outline of Final Research Achievements

N-glycosylation pattern of IgG in patients with rheumatoid arthritis is reduced galactosylation. This aberrant glycosylation improve after TNF-alfa blockade therapy. Glycosylation of IgG is important in effector function of IgG such as interaction with Fc receptor. We purify serum IgG in patients who recieved IL-6 blockade or DMARDs. And we performed glycan analysis using MALDI-TOF MS. The aberrant glycosylation of IgG improve after each therapy.

Academic Significance and Societal Importance of the Research Achievements

今回は少数例の関節リウマチ患者において治療前後のIgG糖鎖の変化を解析した。今後症例数を増やして解析を行う必要がある。問題点としては、今回糖鎖解析に外注を利用したが、輸送費・解析費用など費用面での問題がある。
またサイトカインが糖鎖合成酵素発現に与える影響をin vitroで明らかにしようとしたが、十分なデータを得ることができなかった。

Research Products

• [Journal Article] 関節リウマチにおける糖鎖制御機構 (2017)
  • Author(s): 伊藤清亮、山田和徳
  • Journal Title: アレルギーの臨床 Volume: 37(5) Pages: 501-505
  • Peer Reviewed