| Project/Area Number |
15K19589
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
Yoshida Haruno 北里大学, 感染制御科学府, 助手 (70563386)
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| Research Collaborator |
TAKAHASHI Takashi 北里大学, 感染制御科学府, 教授 (00292855)
OKADA Nobuhiko 北里大学, 薬学部, 教授 (80194364)
ISHIGAKI Yasuhito 金沢医科大学, 総合医学研究所, 教授 (20232275)
MATSUI Hidenori 北里研究所, 生命科学研究所, 講師 (30219373)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | A群レンサ球 / GAS / マウス感染モデル / A群溶血性レンサ球菌 / 感染実験 / 劇症型レンサ球菌感染症 / 転写調節因子 / 比較ゲノム / 臨床分離株 |
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| Outline of Final Research Achievements |
In this research, we aimed to clarify the new mechanism of sever GAS pathogenicity caused by rocA gene mutation.Two clinically isolates named MTB 313 and MTB314, have almost the same genomic structure, but the sequences of the regulator rocA genes are different. Based on this, recombinant GAS was prepared, and the gene expression profiles and pathological conditions were compared.As a result, MTB 313 with rocA mutation showed high lethality to hCD46Tg mice, and expression of many known pathogenic factors was elevated. In contrast, MTB 314 showed little lethality to mice.Summarizing the results, it was suggested that the spontaneous mutation of the rocA gene may have an effect on MTB 313's high pathogenicity.
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