| Project/Area Number |
15K19697
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Oita University |
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Principal Investigator |
Yano Hiroyuki 大分大学, 全学研究推進機構, 教務職員 (50448552)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 放射線誘起線維化 / miRNA / コラーゲン / 放射線誘発線維症 / 細胞外マトリックス / microRNA |
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| Outline of Final Research Achievements |
Radiation-induced fibrosis (RIF) is thought to involve the excessive accumulation of collagen; previously, we reported that radiation increased the type I collagen expression and TGF-β was involved in this increase. It has been suggested that miRNA negatively regulate the gene expression. However, their role in the RIF process remains unclear. In this study, we examined the effects of miRNA on the type I collagen expression induced by radiation. We found that miR-29 were downregulated and targeted type I collagen gene in irradiated cells. We also found that the overexpression of miR-29 inhibited the type I collagen expression whereas the knockdown of miR-29 enhanced it. In addition, TGF-βsignaling decreased the miR-29 expression whereas the inhibition of this signaling cancelled this decrease. In conclusion, miR-29 was involved in the regulation of type I collagen expression through the TGF-β signaling in irradiated cells, suggesting that miR-29 may be an important regulator of RIF.
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