Exploring new combination of radiotherapy and LW6, a novel HIF-1 inhibitor; suprresion of EMT by LW6
| Project/Area Number |
15K19764
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Hirosaki University |
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Principal Investigator |
Sato Mariko 弘前大学, 医学部附属病院, 助教 (30645263)
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| Research Collaborator |
TAKAI Yoshihiro
HIROSE Katsumi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 上皮間葉転換（ＥＭＴ） / ＨＩＦ－１ / ＨＩＦ－１阻害剤 / 低酸素 / 放射線治療 / 上皮間葉転換 / HIF-1α / HIF-1阻害剤 / ＨＩＦ-1阻害剤 / 創傷治癒アッセイ |
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| Outline of Final Research Achievements |
Recently study reported that hypoxic cancer cells are promoted epitherial-mesenchymal transition (EMT) by radiation therapy.The induction of hypoxia-induced EMT involvement of HIF-1 pathway has been suggested, therefore it is expected that LW6 improve the radiotherapeutic effect of hypoxic tumor via HIF-1 inhibition.
The human lung adenocarcinoma A549 cells were cultured under normoxia or hypoxia (O2 1%), and X-irradiated 0 or10 Gy. We performed wound-healing assay and evaluated the expression of EMT markers, JNK and p-JNK. In this study, we revealed that LW6 suprress each of hypoxia- and radiation-induced EMT leading to cancer relapse through HIF-1 pathway and JNK pathway blockade.
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Report
(4 results)
Research Products
(1 results)
