Study of the pathogenesis of early brain injury after subarachnoid hemorrhage
| Project/Area Number |
15K19962
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Mie University |
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Principal Investigator |
Fujimoto Masashi 三重大学, 医学部附属病院, 診療等従事者 (00437104)
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| Research Collaborator |
SUZUKI Hidenori
YOSHIDA Toshimichi
YOSHIDA Kyoko
LIU Lei
NISHIKAWA Hirofumi
NAKANO Fumi
NAKATSUKA Yoshinari
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | くも膜下出血 / 脳損傷 / アポトーシス / 炎症 / 細胞外マトリックス / ノックアウトマウス / テネイシンC |
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| Outline of Final Research Achievements |
Tenascin-C (TNC), a matricellular protein, is upregulated in brain parenchyma after experimental subarachnoid hemorrhage (SAH). The aim of this study was to investigate effects of TNC knockout (TNKO) on neuronal apoptosis and neuroinflammation, both of which are important constituents of early brain injury (EBI) after SAH. C57BL/6 wild-type mice or TNKO mice underwent sham or filament perforation SAH modeling. Deficiency of TNC significantly alleviated post-SAH neurobehavioral impairments and neuronal apoptosis. The protective effects of TNKO on neurons were associated with the inhibition of a caspase-dependent apoptotic pathway, which was at least partly mediated by Toll-like receptor 4 /nuclear factor-κB /interleukins-1β and -6 signaling cascades. This study first provided the direct evidence that TNC causes post-SAH neuronal apoptosis and neuroinflammation, potentially leading to the development of a new molecular targeted therapy against EBI.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Fujimoto M, Shiba M, Kawakita F, Liu L, Shimojo N, Imanaka-Yoshida K, Yoshida T, Suzuki H.2017
Author(s)
Fujimoto M, Shiba M, Kawakita F, Liu L, Shimojo N, Imanaka-Yoshida K, Yoshida T, Suzuki H.
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Journal Title
Mol Neurobiol.
Volume: -
Issue: 3
Pages: 1951-1958
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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