Integrated analysis of metabolomics and genetics using cerebrospinal fluid of glioma patients

• Project/Area Number: 15K19970
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Kobe University
• Principal Investigator: Nakamizo Satoshi 神戸大学, 医学研究科, 医学研究員 (00569238)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: メタボローム解析 / グリオーマ / IDH変異 / 2HG / Cerebrospinal fluid / microRNA / metabolome

Outline of Final Research Achievements

Using gas chromatography/mass spectorometry, metabolome analysis of cerebrospinal fluid of 53 patients was performed。And isocitrate dehydrogenase (IDH) mutation of the tumor was examined. The mean concentration of 2-hydroxygulitarate (2HG) in the CSF was 0.48mM. The mean concentration of 2HG in glioblastoma, anaplastic astrocytoma, diffuse astrocytoma, and olifodendroglioma were 0.41mM, 0.46 mM, 0.71 mM, and 0.49 mM, respectively. There was no significant difference among tumor pathology. There were 11 cases with IDH mutation. In IDH mutation group, the mean concentration of 2HG was 0.61±0.2mM, on the other hand, in IDH wild-type group, the mean concentration of 2HG was 0.44±0.06mM. There was no significant difference of 2HG concentration between IDH mutation group and IDH wild-type group. These results indicate that 2HG concentration of CSF is not useful biomarker for the glima patients.

Research Products

• [Journal Article] Radiographic occult cerebellar germinoma presenting with progressive ataxia and cranial nerve palsy. (2016)
  • Author(s): Minami N, Tanaka K, Kimura H, Hirose T, Mori T, Maeyama M, Sekiya H, Uenaka T, Nakamizo S, Nagashima H, Mizukawa K, Itoh T, Sasayama T, Kohmura E.
  • Journal Title: BMC Neurol. Volume: 16 Issue: 1 Pages: 1-4
  • DOI: 10.1186/s12883-015-0516-9
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL) (2015)
  • Author(s): Sasayama T, Tanaka K, Mizowaki T, Nagashima H, Nakamizo S, Tanaka H, Nishihara M, Mizukawa K, Hirose T, Itoh T, Kohmura E
  • Journal Title: Brain Pathol. Volume: Aug 28 Issue: 4 Pages: 1-9
  • DOI: 10.1111/bpa.12318
  • Peer Reviewed
• [Journal Article] A 40-Year-Old Female with Leptomeningeal Lesions. (2015)
  • Author(s): Mizowaki T, Sasayama T, Semba S, Sasaki R, Yasuo K, Nakamizo S, Tanaka K, Mizukawa K, Uchihashi Y, Kohmura E.
  • Journal Title: Brain Pathol. Volume: 25 Issue: 4 Pages: 509-10
  • DOI: 10.1111/bpa.12270
  • Peer Reviewed / Open Access
• [Journal Article] STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma. (2015)
  • Author(s): Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E
  • Journal Title: J Neurooncol Volume: 124(2) Issue: 2 Pages: 165-74
  • DOI: 10.1007/s11060-015-1843-9
  • Peer Reviewed
• [Presentation] 破裂脳動脈瘤に対するコイル塞栓術後２日目に後腹膜血腫を来した１例 (2017)
  • Author(s): 中村直人, 中溝聡、阪上義雄
  • Organizer: 第42回日本脳卒中学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2017-03-16
• [Presentation] 80歳以上の高齢者頚動脈狭窄症に対する頚動脈ステント留置術(CAS)の長期予後 (2017)
  • Author(s): 阪上義雄、中溝聡
  • Organizer: 第42回日本脳卒中学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2017-03-16