The development of a novel biomarker using expression profiles of exosomal microRNAs in CSF
| Project/Area Number |
15K19975
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Ehime University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | グリオーマ / microRNA / エクソソーム |
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| Outline of Final Research Achievements |
In the present study, we aimed to profile the expression of exosomal microRNAs in CSF and develop a novel biomarker. The exosomal microRNAs were extracted from the CSF of five glioblastoma patients and three non-tumoral volunteers(NPH). Using microRNA microarray, we comprehensively analyzed the 2,006 microRNAs expression profiles.
107 microRNAs (57 up-regulated and 50 down-regulated microRNAs) were aberrantly expressed in glioblastoma patients compared with non-tumoral volunteers. In addition, we defined 3 microRNAs as a Glioma-initiating cells specific exoxomal microRNA that were aberrantly expressed in Glioma-initiating cells and glioblastoma patients compared with neuroral stem cells and healthy volunteers.
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Report
(3 results)
Research Products
(4 results)
