Elucidation of novel bone matrix regulatory system by collagens VI and XII.

• Project/Area Number: 15K19988
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Chiba Institute of Science (2016); Tokyo Medical and Dental University (2015)
• Principal Investigator: Izu Yayoi 千葉科学大学, 危機管理学部, 講師 (90431949)
• Research Collaborator: AIDA Tomomi Massachusetts Institute of Technology, Research Scientist ; BIRK David E. University of South Florida, Professor ; KOCH Manuel University of Cologne
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: VI型コラーゲン / XII型コラーゲン / 骨形成 / 骨芽細胞 / 骨芽細胞ネットワーク / 骨の質の制御 / 骨芽細胞コミュニケーションネットワーク / 細胞間コミュニケーションネットワーク

Outline of Final Research Achievements

In this research, we aimed to elucidate the control mechanism of bone quality, focusing on regulation of bone forming osteoblast cell-cell communication. We have elucidated that lack of collagen VI or collagen XII induces osteoblast shape change thereby decreased bone quality. In this research, we found that cooperative regulation of collagens VI and XII form osteoblast cell-cell communication network.

Research Products

• [Int'l Joint Research] University of South Florida/University of Pennsylvania/University of California(米国)
• [Int'l Joint Research] University of Cologne(Germany)
• [Int'l Joint Research] University of South Florida(米国)
• [Int'l Joint Research] University of Cologne(Germany)
• [Journal Article] Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis. (2016)
  • Author(s): Izu Y., Ezura Y., Koch M., Birk DE., Noda M.
  • Journal Title: Cell Tissue Res. Volume: 364 Issue: 3 Pages: 623-635
  • DOI: 10.1007/s00441-015-2345-y
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ. (2016)
  • Author(s): Aida T, Nakade S, Sakuma T, Izu Y, Oishi A, Mochida K, Ishikubo H, Usami T, Aizawa H, Yamamoto T, Tanaka K.
  • Journal Title: BMC Genomics Volume: 17 Issue: 1 Pages: 979-979
  • DOI: 10.1186/s12864-016-3331-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis. (2016)
  • Author(s): Yayoi Izu, Yoich Ezura, Manuel Koch, David E. Birk, Masaki Noda
  • Journal Title: Cell Tissue Research Volume: 未定
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interleukin-1β Suppresses the Transporter Genes Ank and Ent1 Expression in Stromal Progenitor Cells Retaining Mineralization. (2016)
  • Author(s): Yoich Ezura, Xin Lin, Arina Hatta, Yayoi Izu, Masaki Noda
  • Journal Title: Calcif Tissue Int. Volume: 未定
  • Peer Reviewed
• [Journal Article] Cathepsin K Deficiency Suppresses Disuse-Induced Bone Loss. (2016)
  • Author(s): Moriya S, Izu Y, Arayal S, Kawasaki M, Hata K, Pawaputanon Na Mahasarakhahm C, Izumi Y, Saftig P, Kaneko K, Noda M, Ezura Y.
  • Journal Title: J Cell Physiol. Volume: 231 Issue: 5 Pages: 1163-1170
  • DOI: 10.1002/jcp.25214
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mice Deficient in CIZ/NMP4 Develop an Attenuated Form of K/BxN-Serum Induced Arthritis. (2016)
  • Author(s): Nakamoto T, Izu Y, Kawasaki M, Notomi T, Hayata T, Noda M, Ezura Y.
  • Journal Title: J Cell Biochem. Volume: 117 Issue: 4 Pages: 970-977
  • DOI: 10.1002/jcb.25382
  • Peer Reviewed / Open Access
• [Journal Article] BMP-2 Enhances Lgr4 Gene Expression in Osteoblastic Cells. (2016)
  • Author(s): Pawaputanon Na Mahasarakham C, Ezura Y, Kawasaki M, Smriti A, Moriya S, Yamada T, Izu Y, Nifuji A, Nishimori K, Izumi Y, Noda M.
  • Journal Title: J Cell Physiol. Volume: 231 Issue: 4 Pages: 887-895
  • DOI: 10.1002/jcp.25180
  • Peer Reviewed
• [Journal Article] Profilin Expression Is Regulated by Bone Morphogenetic Protein (BMP) in Osteoblastic Cells. (2016)
  • Author(s): Lin W, Ezura Y, Izu Y, Aryal A C S, Kawasaki M, Na Mahasarakham Chantida P, Moriyama K, Noda M.
  • Journal Title: J Cell Biochem. Volume: 117 Issue: 3 Pages: 621-628
  • DOI: 10.1002/jcb.25310
  • Peer Reviewed
• [Journal Article] Beta Adrenergic Receptor Stimulation Suppresses Cell Migration in Association with Cell Cycle Transition in Osteoblasts-Live Imaging Analyses Based on FUCCI System. (2016)
  • Author(s): Katsumura S, Ezura Y, Izu Y, Shirakawa J, Miyawaki A, Harada K, Noda M.
  • Journal Title: J Cell Physiol. Volume: 231 Issue: 2 Pages: 496-504
  • DOI: 10.1002/jcp.25096
  • Peer Reviewed
• [Journal Article] Nck influences preosteoblastic/osteoblastic migration and bone mass (2015)
  • Author(s): Aryal Smriti, Kentaro Miyai, Yayoi Izu, Tadayoshi Hayata, Takuya Notomi, Masaki Noda, Yoichi Ezura
  • Journal Title: Proc Natl Acad Sci USA Volume: 112 Issue: 50 Pages: 15432-7
  • DOI: 10.1073/pnas.1518253112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Accumulation of type VI collagen in the primary osteon of the rat femur during postnatal development. (2015)
  • Author(s): Kohara Y, Soeta S, Izu Y, Amasaki H.
  • Journal Title: J Anat. Volume: 226 Issue: 5 Pages: 478-488
  • DOI: 10.1111/joa.12296
  • Peer Reviewed
• [Journal Article] TGF-β Suppresses Ift88 Expression in Chondrocytic ATDC5 Cells. (2015)
  • Author(s): Kawasaki M, Ezura Y, Hayata T, Notomi T, Izu Y, Noda M.
  • Journal Title: J Cell Physiol. Volume: 230 Pages: 2788-2795
  • Peer Reviewed
• [Presentation] Collagen XII regulates cellular compartmentalization in tendon that mediates initiation of fibrillogenesis. (2016)
  • Author(s): Yayoi Izu, Qingmei Yao, Chinda Hemmavanh, Sheila M. Adams, Thomas H. Adams, Brianne K. Connizzo, David P. Beason, James V. Jester, Louis J. Soslowsky, Manuel Koch and David E. Birk
  • Organizer: American Society for Bone Matrix 2016
  • Place of Presentation: Hilton St. Petersburg Bayfront, Florida（USA)
  • Year and Date: 2016-11-13
  • Int'l Joint Research
• [Presentation] XII型コラーゲンは腱細胞の細胞間コミュニケーションネットワークに関与する (2016)
  • Author(s): 伊豆 弥生 、David E. Birk
  • Organizer: 第３４回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] Collagens VI and XII Matrix Bridges Mediate Osteoblast Cell Communicating Networks During Bone Formation (2015)
  • Author(s): Yayoi Izu, Yoichi Ezura, Manuel Koch, David E. Birk, Masaki Noda
  • Organizer: The American Society for Bone and Mineral Research 2015 annual meeting
  • Place of Presentation: Seattle（USA）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] VI型およびXII型コラーゲン複合体による骨芽細胞間コミュニケーションネットワークの制御 (2015)
  • Author(s): 伊豆 弥生、江面 陽一、野田 政樹
  • Organizer: 第33回 日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル（東京都、新宿）
  • Year and Date: 2015-07-23
• [Presentation] VI型およびXII型コラーゲン複合体が骨形成過程における骨芽細胞間コミュニケーションネットワークを制御する (2015)
  • Author(s): 伊豆 弥生、江面 陽一、Manuel Koch 、 David E. Birk 、野田 政樹
  • Organizer: 第３５回 日本骨形態計測学会
  • Place of Presentation: 倉敷市芸文館（岡山県、倉敷市）
  • Year and Date: 2015-06-04
• [Presentation] Collagens VI and XII form complexes mediating osteoblast interaction during osteogenesis. (2015)
  • Author(s): Yayoi Izu, Yoichi Ezura, Manuel Koch, David E. Birk, Masaki Noda
  • Organizer: 13th Congress of the International Society of Bone Morphometry
  • Place of Presentation: 東京ガーデンパレスホテル（東京都、文京区）
  • Year and Date: 2015-04-27
  • Int'l Joint Research