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Analysis of cancer-specific urinary exosomal miRNA derived from urothelial carcinoma

Research Project

Project/Area Number 15K20089
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

Matsuzaki Kyosuke  大阪大学, 医学部附属病院, 医員 (90747081)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords尿路上皮癌 / エクソソーム / 尿中 / バイオマーカー / 尿中エクソソーム
Outline of Final Research Achievements

Exosomes were extracted from urine of 6 urothelial carcinoma (UC) patients and 3 healthy volunteers by ultracentrifugation, and miRNA from urinary Exosomes was analyzed by miRNA array. We identified 5 miRNAs (miR-155-5p, miR-15a-5p, miR-21-5p, miR-132-3p and miR-31-5p) overexpressed in urinary exosomes of UC patients from miRNA microarray analysis.Five candidate miRNAs were all significantly expressed more highly in urinary exosomes of UC patients compared to those of the control in verification cohort (N=60, all P<0.0001). Of the 5 miRNAs, miR-21-5p in urinary exosomes was the most potent biomarker, having a sensitivity of 75.0%, specificity of 95.8%. This result was better than that of urine cytology in this cohort.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] MiR-21-5p in urinary extracellular vesicles is a novel biomarker of urothelial carcinoma.2017

    • Author(s)
      Matsuzaki K et al.
    • Journal Title

      Oncotarget

      Volume: - Issue: 15 Pages: 14969-14969

    • DOI

      10.18632/oncotarget.14969

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 尿中エクソソーム内miR-21-5pは尿路上皮癌のバイオマーカーになり得る2017

    • Author(s)
      松崎恭介、藤田和利、山本致之 、木内利郎 、林 拓自、川村憲彦、河嶋厚成、氏家剛、永原啓、植村元秀、神宮司健太郎、辻川和丈、野々村祝夫
    • Organizer
      第105回日本泌尿器科学会総会
    • Place of Presentation
      白山観光ホテル
    • Related Report
      2016 Annual Research Report
  • [Presentation] 尿路上皮癌由来の尿中エクソソームmiRNA解析および新規バイオマーカーの探索2016

    • Author(s)
      松崎恭介、藤田和利、山本致之 、木内利郎 、林 拓自、川村憲彦、河嶋厚成、氏家剛、永原啓、植村元秀、神宮司健太郎、辻川和丈、野々村祝夫
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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