Analysis of cancer-specific urinary exosomal miRNA derived from urothelial carcinoma
| Project/Area Number |
15K20089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 尿路上皮癌 / エクソソーム / 尿中 / バイオマーカー / 尿中エクソソーム |
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| Outline of Final Research Achievements |
Exosomes were extracted from urine of 6 urothelial carcinoma (UC) patients and 3 healthy volunteers by ultracentrifugation, and miRNA from urinary Exosomes was analyzed by miRNA array. We identified 5 miRNAs (miR-155-5p, miR-15a-5p, miR-21-5p, miR-132-3p and miR-31-5p) overexpressed in urinary exosomes of UC patients from miRNA microarray analysis.Five candidate miRNAs were all significantly expressed more highly in urinary exosomes of UC patients compared to those of the control in verification cohort (N=60, all P<0.0001). Of the 5 miRNAs, miR-21-5p in urinary exosomes was the most potent biomarker, having a sensitivity of 75.0%, specificity of 95.8%. This result was better than that of urine cytology in this cohort.
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Report
(3 results)
Research Products
(3 results)
