| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
IL-33 and sST2 have been reported as key regulators upon decidualization. IL-33 is processed into active forms by cathepsin G (CTSG). In this study, we investigated the existence and contribution of IL-33, sST2 and CTSG in early pregnancy of patients with recurrent miscarriage. With informed consent, cervical mucus and decidua were surgically collected. Immunohistochemistry and ELISA were performed using antibodies against IL-33, sST2 and CTSG.
Staining of IL-33 and CTSG was observed in the cytoplasm of stromal and epithelial cells in decidua. The expression of CTSG in cervical mucus of patients with spontaneous miscarriage was significantly higher than that of successful pregnancy. CTSG expression is especially high in miscarriage group when they are early gestational age (4-5week) and when the fetal chromosome is normal. IL-33 and ST2 were not significantly different in both groups. The results provided that CTSG plays an important role in the mechanism of early pregnancy.
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