| Project/Area Number |
15K20156
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 胎盤 / 絨毛外栄養膜細胞 / カルレティキュリン / 分子シャペロン / 妊娠高血圧症候群 |
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| Outline of Final Research Achievements |
The expression of Calreticulin (CRT) was detected in four trophoblastic cell lines and human placenta of normal pregnant women which was obtained with informed consent. In the human extravillous trophoblast (EVT) cell line HTR8/SVneo cells, CRT knockdown suppressed the invasion ability and the adhesion to fibronectin, and suppressed the phosphorylation level of Akt on attachment to fibronection. CRT knockdown modulated N-glycosylation of integrin (Itg) β1, and changed the expression level of several glycosyltransferases in the cells. These results showed that CRT, which interacts with the glycosylated protein, promotes cell adhesion and invasion ability of EVTs by regulating N-glycosylation of Itg β1.
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