Involvement of transcription factor HOXD9 in the malignant phenotype of cervical cancer
| Project/Area Number |
15K20159
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
SUGA Yukako 慶應義塾大学, 医学部(信濃町), 助教 (40459558)
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| Research Collaborator |
KAWAKMI Yutaka 慶應義塾大学, 医学部, 教授 (50161287)
IWATA Takashi 慶應義塾大学, 医学部, 講師 (30296652)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | HOXD9 / P97プロモーター / 子宮頸癌 / HPV陰性腺癌 / P53 |
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| Outline of Final Research Achievements |
Expression changes of all genes by suppressing HOXD9 in cervical cancer cell lines SKG-3B were examined by DNA chip.Based on IPA analysis, P53 signaling was activated after suppression of HOXD9 expression in SKG-3B cells. P53 gene expression did not change, while the P53 protein level was elevated in HOXD9 supressed SKG3B and Siha cells. Luciferase activity was significantly decreased after HOXD9 expression was suppressed in SKG-IIIB cells. In the CHIP assay in SiHa cells (HPV16 positive), HOXD9 was shown to directry bind to the P97 promoter.
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Report
(4 results)
Research Products
(1 results)
