• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Relationship between lutenization function and Calpastatin

Research Project

Project/Area Number 15K20167
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionInternational University of Health and Welfare (2018)
St. Marianna University School of Medicine (2015-2017)

Principal Investigator

Kawashima Ikko  国際医療福祉大学, 医学部, 研究員 (40633946)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords黄体化 / 更年期障害 / 排卵 / Calpain / 顆粒層細胞 / Calpastatin / カルシウム / PRDM1 / 卵巣 / 生殖細胞 / 髄質 / カルパスタチン / PRDM1-Cre / 黄体 / 閉経
Outline of Final Research Achievements

Our study suggested that luteinization requires the activity of Calpain at 5.5 hours after LH stimulation. It was found that when Calpain activity in Granulosa cells was inhibited during luteinization, luteal cells did not become normal progesterone-producing cells but became Estrogen-producing cells. Since this is a phenotype observed in the early stage of the menopausal disorder, We considered that Calpain activity decreased early in menopause. And our considered that calpastatin, as endogenous calpain inhibitor, was blocked calpain activation. The expression of Calpastatin was detected in aged mice and young mice, and it was clarified that it was predominantly high in aged mice. Furthermore, another group showed that the Calpain 1/2 knockout mice had normal fertility. Therefore, Calpain family (Calpain 1/2 and more) activation induced luteinization from granulosa cells and overexpression of Calpastatin in aged mice may block luteinization.

Academic Significance and Societal Importance of the Research Achievements

LH刺激による黄体化は、遺伝子発現によって制御を受けていることが知られていたが、タンパク質の活性が、その細胞の分化を制御していることを示唆することができた。さらに、更年期障害の初期でみられるエストロジェン過多を誘発する可能性が示唆される知見が得られ、排卵刺激後のどの時間でそのイベントが発生しうるかを示すことができた。現在、どのCalpainが重要な機能を示しているかを検証する必要はあるが、更年期障害を緩和するための新たな分子標的が明らかとなったことに社会的意義がある。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results)

  • [Journal Article] Regulation of follicle growth through hormonal factors and mechanical cues mediated by Hippo signaling pathway.2018

    • Author(s)
      Kawashima I, Kawamura K.
    • Journal Title

      Syst Biol Reprod Med.

      Volume: 64 Issue: 1 Pages: 3-11

    • DOI

      10.1080/19396368.2017.1411990

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heterosis extends the reproductive ability in aged female mice†2018

    • Author(s)
      Ozawa Yusuke、Watanabe Kenji、Toda Toshihiko、Shibuya Shuichi、Okumura Nobuaki、Okamoto Naoki、Sato Yorino、Kawashima Ikko、Kawamura Kazuhiro、Shimizu Takahiko
    • Journal Title

      Biology of Reproduction

      Volume: 100 Issue: 4 Pages: 1082-1089

    • DOI

      10.1093/biolre/ioy260

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The acceleration of reproductive aging in Nrg1flox/flox ;Cyp19-Cre female mice.2017

    • Author(s)
      Umehara T, Kawai T, Kawashima I, Tanaka K, Okuda S, Kitasaka H, Richards JS, Shimada M.
    • Journal Title

      Aging Cell.

      Volume: 16 Issue: 6 Pages: 1288-1299

    • DOI

      10.1111/acel.12662

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] isorganization of the germ cell pool leads to primary ovarian insufficiency.2017

    • Author(s)
      Kawashima I, Kawamura K
    • Journal Title

      Reproduction

      Volume: 印刷中 Issue: 6 Pages: R205-R213

    • DOI

      10.1530/rep-17-0015

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Neuregulin 1 regulates proliferation of leydig cells to support spermatogenesis and sexual behavior in adult mice2016

    • Author(s)
      Umehara T, Kawashima I, Kawai T, Hoshino Y, Morohashi KI, Shima Y, Richards JS, Shimada M
    • Journal Title

      Endocrinology

      Volume: 157 Issue: 12 Pages: 4899-4913

    • DOI

      10.1210/en.2016-1478

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi