• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of role of CDKN3 in taxan resistant human head and neck cancer

Research Project

Project/Area Number 15K20195
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionUniversity of Fukui

Principal Investigator

Ito Yumi  福井大学, 学術研究院医学系部門(附属病院部), 特命助教 (00646458)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsCDKN3 / タキサン耐性 / 頭頸部癌 / 頭頚部扁平上皮癌 / タキソール耐性
Outline of Final Research Achievements

We established paclitaxel-resistant cancer cell line T3M-1 TX, from T3M-1 human oropharyngeal cancer cell line. T3M-1TX cells have docetaxel resistance in addition to paclitaxel. T3M-1TX cells show that paclitaxel treatment induces G1/S arrest and G2/M transition of the cell cycle. In the result of PCR array, we focused on Cyclin dependent kinase inhibitor 3 (CDKN3), a regulator of G1/S arrest and G2/M transition. The specific siRNA for CDKN3 reduced the expression of CDKN3, resulting in enhanced apoptosis after paclitaxel and docetaxel treatment. The MTT assays and clonogenic cell survival assays proved that inhibition of CDKN3 reduced cell viability after paclitaxel and docetaxel treatment. CDKN3 is a novel molecular target to overcome taxane-resistance of head and neck cancers.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2016

All Presentation (2 results)

  • [Presentation] 福井県における新生児聴覚スクリーニング後の2次精査の現状と問題点について2016

    • Author(s)
      伊藤 有未
    • Organizer
      第61回日本聴覚医学会総会・学術講演会
    • Place of Presentation
      盛岡グランドホテル(岩手県盛岡市)
    • Year and Date
      2016-10-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] FNAにおける新規マーカーの解析ーFFPE標本を用いたPCR array2016

    • Author(s)
      伊藤 有未
    • Organizer
      第40回日本頭頸部癌学会
    • Place of Presentation
      ソニックシティ(埼玉県さいたま市)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi